我国不同艾滋病候选疫苗小鼠细胞免疫应答评价  

Evaluation of cellular immune responses in mice elicited by Chinese AIDS candidate vaccines

作　　者：黄维金[1,2] 张春涛[1] 赵晨燕[1] 聂建辉[1] 宋爱京[1] 鲁凤民[2] 王佑春[1] 

机构地区：[1]中国药品生物制品检定所细胞室,北京100050 [2]北京大学医学部病原微生物学系,100080

出　　处：《中华微生物学和免疫学杂志》2010年第9期838-842,共5页Chinese Journal of Microbiology and Immunology

摘　　要：目的 比较我国不同艾滋病（AIDS）候选疫苗小鼠细胞免疫应答水平.方法 将我国6种艾滋病候选疫苗按其各自免疫程序,免疫BALB/c小鼠,分离脾细胞,利用酶联免疫斑点法（ELISPOT）与胞内因子染色法（ICS）检测其对HIV特异多肽的细胞免疫应答水平.结果 6种艾滋病候选疫苗利用潜在T细胞表位（PTE）Gag、Env和Pol肽库和ELISPOT法检测IFN-γ的分泌情况,结果各疫苗的小鼠细胞免疫阳转率为70%～100%,各疫苗引起特异细胞免疫应答的反应肽库不同,强度亦不相同.1#和2#疫苗利用ELISPOT检测分泌Th1类细胞因子IFN-γ和IL-2的小鼠脾细胞数量,两疫苗的IFN-γ和IL-2检测结果均呈中度相关性（r1=0.62,P1＜0.01;r2=0.79,P2＜0.01）.ELISPOT和ICS检测1#疫苗免疫后分泌IFN-γ的小鼠脾细胞数,结果两种方法的小鼠阳转率均为66.7%（10/15）,ELISPOT阳性细胞数与ICS阳性细胞比例呈中度相关（r=0.55,P＜0.05）.结论 我国不同AIDS候选疫苗引起的小鼠细胞免疫应答在广度和强度上存在差别.ELISPOT或ICS检测IFN-γ等Th1类细胞因子可作为疫苗诱导小鼠细胞免疫应答的评价指标.Objective To compare cellular immune responses in mice elicited by Chinese different AIDS candidate vaccines.Methods According to their different immunization procedures,BALB/c mice were immunized with 6 AIDS candidate vaccines,separately.Spleen cells were isolated for the detection of cellular immune response to HIV-specific peptides using enzyme-linked immunosorbent spot（ELISPOT）assay and intracellular cytokine staining（ICS）method.Results AIDS vaccines were evaluated by using potential T-cell epitopes（PTE）Gag,Env and Pol peptides pool and ELISPOT.The positive conversion rates for cellular immune response of 1#-6# vaccines fluctuated from 70% to 100%.The vaccine-induced cellular immune responses to specific peptides pool are different not only in magnitude but also in breadth.The Th1type cytokines,IFN-γand IL-2,were detected with ELISPOT in 1# and 2# vaccines.The productions of IFN-γand IL-2 induced by both of the two vaccines showed a moderate correlation（r1 =0.62,P1 〈0.01 ;r2=0.79,P2 〈 0.01）.The positive conversion rate of IFN-γ secreting cells of 1 # vaccine was 66.7%（10/15）mice detected with both ELISPOT and ICS.And the results tested by ELISPOT and ICS showed moderate correlation（r = 0.55,P 〈 0.05）.Conclusion The magnitude and breadth of cellular immune responses induced by different AIDS candidate vaccines are different.Being induced by different AIDS candidate vaccines,the IFN-γand other Th1 type cytokines detected by ELISPOT or ICS could be used to evaluate the cellular immune responses in mice.

关 键 词：艾滋病 疫苗 细胞免疫应答 

分 类 号：R392[医药卫生—免疫学]