17α-甲基睾酮诱导尼罗罗非鱼雄性化后在鱼肌肉内残留研究  

Study on 17α-Methyltestosterone residues in muscle tissues of Nile tilapia masculinized by 17α-Methyltestosterone

作　　者：莫媛媛[1,2] 卢迈新[1] 杨丽萍[1] 叶星[1] 朱华平[1] 高风英[1] 黄樟翰[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所,广东广州510380 [2]广东海洋大学水产学院,广东湛江524088

出　　处：《广东农业科学》2010年第10期146-148,共3页Guangdong Agricultural Sciences

基　　金：国家科技支撑计划项目(2006BAD01A1201);广东省农业重点项目(2009B020201003);现代农业产业技术体系建设专项(nycytc-48-4);公益性行业(农业)科研专项(3-49);广东省海洋渔业科技推广专项(A200899B02);广东省重大科技兴渔项目(B200701A06)

摘　　要：采用不同浓度梯度17α-甲基睾酮(17α-Methyltestosterone,MT)饲料(5、10、30、50、60mg/kg)处理孵化后10d的尼罗罗非鱼鱼苗30d,之后改喂正常饲料继续进行养殖。经过4个月的饲喂后,MT处理组的绝对增长率、绝对增重率、瞬时增长率和瞬时增重率等参数均与对照组间存在显著差异(P<0.05)。统计雄性率,发现高浓度处理组(50mg/kg和60mg/kg)超过99%,低浓度处理组(5mg/kg和10mg/kg)则低于80%。MT处理30d后,对照组与处理组MT残留量分别为对照组(0μg/kg)<5mg/kg组(23.19μg/kg)<10mg/kg组(30.82μg/kg)<30mg/kg组(37.76μg/kg)<50mg/kg组(44.75μg/kg)<60mg/kg组(68.41μg/kg)。残留量与MT添加量呈现正相关。继续投喂正常饲料30d后,在各浓度处理组均未检测到MT残留。以上研究结果表明,MT处理30d可提高罗非鱼苗雄性率,促进生长;MT处理后60d,均检测不出MT残留。At 10 days post-fertilization（DPF）,Nile tilapia（Oreochromis niloticus） fry were fed by 17α-Methylte-stosterone（MT）treated diet（0,5,10,30,50 and 60 mg/kg） for 30 days,then the MT-treated diet were replaced by MT-free commercial feed.After four months experiment,the absolute growth rate of length（AGRL）,absolute growth rate of weight（AGRw）,specific growth rate of length（SGRL） and specific growth rate of weight（SGRW） had a significant difference among MT treated groups and control group respectively.In 50 mg/kg and 60 mg/kg MT-treated groups,the male population was more than 99%,while in 5 mg/kg and 10 mg/kgMT-treated groups,the male population was less than 80%.The residue of MT in muscle tissue at 30 days in each group was ranked as control group（0 μg/kg） 5 mg/kg group（23.19 μg/kg）10 mg/kg group（30.82 μg/kg）30 mg/kg group（37.76 μg/kg）50 mg/kg group（44.75 μg/kg）60 mg/kg group（68.41 μg/kg）.However,the residue of MT was not detected in any treated group at 60 days.The results indicated that the MT acted as sex-inversion agents by functionally masculinizing Nile tilapia and improved the fish growth rate.After administering MT-free commercial feed for 30 days,the residue of MT could not be detected in muscle tissue.

关 键 词：尼罗罗非鱼 甲基睾酮 残留 性转化 

分 类 号：S816.73[农业科学—饲料科学]