清开灵注射液不同时间给药对MCAO小鼠血清SOD的影响  

The Effect of Qingkailing Injection on Plasma SOD in Different Time Following Middle Cerebral Artery Occlusion

作　　者：程发峰[1] 郭少英[1] 钟相根[1] 鲁艺[1] 宋文婷[2] 王庆国[1] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]中国中医科学院西苑医院,北京100091

出　　处：《中华中医药学刊》2010年第10期2059-2061,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(90709051);重大新药创制科技重大专项(2009ZX09102-136)

摘　　要：目的:研究清开灵注射液不同时间给药对MCAO小鼠血清SOD的影响。方法:将60只昆明小鼠随机分为6组:假手术组、模型组、0h给药组、1h给药组、3h给药组、4h给药组,每组10只。线栓法制备局灶脑缺血模型,采用C lark评分方法进行神经功能评分,用TTC法测定脑梗塞体积。采用黄嘌呤氧化酶法测定SOD活力。结果:缺血同时及缺血后1h、3h、4h给予清开灵注射液治疗,均能显著减少梗塞体积,局灶功能损伤均有显著恢复。清开灵注射液不同时间点给药,均可显著增加血清SOD的含量,并且1h给药时作用最明显。结论:清开灵注射液对脑缺血再灌小鼠有保护作用,抗氧化作用是其机制之一。Objective： To study the effect of Qingkailing（QKL） injection on plasma SOD following middle cerebral artery occlusion.Methods： Sixty Kunming mice were randomly and evenly divided into a sham operation group,model group,0h administration groups,1h administration groups,3h administration groups and 4h administration groups.Cerebral ischemia model of middle cerebral artery occlusion was established in rats using the suture method.Neurological deficit evaluation,such as Clark scoring method,was then carried out at 24 hours after MCAO and cerebral infarction volume was examined after TTC staining.Results： The results showed that cerebral infarction volume reduced significantly after QKL injection administration at time 0h,1h,3h,4h.Local cerebral injury recovered significantly.The content of plasma SOD both increased significantly after QKL administration in different time,and the effect of 1h administration group was the best.Conclusion：The protection of QKL injection was demonstrated on MACO model and antioxidation was one of the mechanisms.

关 键 词：MCAO 清开灵注射液 时间点 血清SOD 

分 类 号：R285.5[医药卫生—中药学]