高压氧对大鼠继发性脊髓损伤保护作用的实验研究  

Protective effects of hyperbaric oxygen in secondary spinal cord injury

作　　者：刘芳[1] 陈虹[2] 苏华[1] 陈佳[1] 

机构地区：[1]福建医科大学附属第一医院高压氧治疗科,福州350005 [2]福建医科大学附属第一医院病理科,福州350005

出　　处：《中华物理医学与康复杂志》2010年第9期649-652,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：基金项目：福建省自然科学基金计划资助项目（X0750030）

摘　　要：目的探讨高压氧（HBO）治疗对继发性脊髓损伤（SCI）的保护作用及其机制。方法96只SD大鼠采用Allen打击法造成SCI后分为HBO组、常氧高压氮组、常压吸氧组和常压空气组，分别于损伤后第1，3，7，14天取材，采用原位末端标记法（TUNEL）检测凋亡细胞，PV-9000二步法免疫组化检测caspase-3表达，光镜下观察，并对结果进行统计学分析；神经功能评价采用BBB评分。结果HBO组在各时间点easpase-3表达均低于其他各组；TUNEL结果亦显示，HBO组凋亡细胞数低于其他各组；HBO组大鼠神经功能明显改善。结论HBO能通过下调caspase-3的表达抑制SCI后细胞凋亡，其作用与氧有关。Objective To investigate the mechanism underlying the protective effect of hyperbaric oxygen （HBO） in secondary spinal cord injury （SCI）. Methods Models of acute SCI were established in 96 Sprague- Dawley rats using Allen＇s dropping weight technique. The rats were then divided into a HBO group, a l^igh pressure nitrogen normal oxygen group, a normal pressure oxygen group and a normal pressure air group. The injured spinal cords were sampled for morphological studies at the 1 st, 3rd, 7th and 14th day after injury. Apoptotic cells were la- beled using the TUNEL technique, and the expression of caspase-3 was detected. The neurological functionality of the spinal cord was assessed by open field locomotor evaluation （the BBB score）. Results The expression of caspase- 3 in the HBO group decreased significantly more than in the other groups after injury. The number of TUNEL-positive cells was significantly lower in the HBO group as well. Neurological function improved significantly after HBO thera- py. Conclusions HBO can down-regulate the expression of caspase-3 and inhibit cell apoptosis in rats after SCI. The protective effect of HBO was related with the oxwen level.

关 键 词：高压氧 脊髓损伤 凋亡 

分 类 号：R651.2[医药卫生—外科学]