cDNA cloning of conotoxins with framework XII from several Conus species  

作　　者：Zhuguo Liu Zheng Yu Na Liu Chongjia Zhao Jie Hu Qiuyun Dai 

机构地区：[1]Beijing Institute of Biotechnology, Beijing 100071, China

出　　处：《Acta Biochimica et Biophysica Sinica》2010年第9期656-661,共6页生物化学与生物物理学报（英文版）

摘　　要：In our efforts for cloning novel I2-superfamily conotoxins using the signal peptide sequence, we identified a novel conotoxin Lt12.4 from Conus litteratus. This gene has a framework XII （-C-C-C-C-CC-C-C-）, which is distinct from the cysteine pattern I2-superfamily conotoxin （-C-C-CC- CC-C-C-）. Subsequently, we found the signal peptide sequence of Lt12.4 by 5′-RACE. Using this new sequence, we identified another five novel conotoxins with this cysteine pattern from four Conus species （Conus eburneus, Conus imperialis, Conus marmoreus, and C. litteratus）. These novel conotoxins have the same cysteine pattern as the reported GIa-TxX and GIa-MII, and may contain Gla residues. Furthermore, they have the highly conserved signal peptide and hypervariable mature peptide sequences, and widely exist in Conus species. Therefore, it could be defined as a new superfamily of E-conotoxins.In our efforts for cloning novel I2-superfamily conotoxins using the signal peptide sequence, we identified a novel conotoxin Lt12.4 from Conus litteratus. This gene has a framework XII （-C-C-C-C-CC-C-C-）, which is distinct from the cysteine pattern I2-superfamily conotoxin （-C-C-CC- CC-C-C-）. Subsequently, we found the signal peptide sequence of Lt12.4 by 5′-RACE. Using this new sequence, we identified another five novel conotoxins with this cysteine pattern from four Conus species （Conus eburneus, Conus imperialis, Conus marmoreus, and C. litteratus）. These novel conotoxins have the same cysteine pattern as the reported GIa-TxX and GIa-MII, and may contain Gla residues. Furthermore, they have the highly conserved signal peptide and hypervariable mature peptide sequences, and widely exist in Conus species. Therefore, it could be defined as a new superfamily of E-conotoxins.

关 键 词：framework XII CONOPEPTIDES cDNA clone CONUS E-conotoxins 

分 类 号：Q78[生物学—分子生物学] TQ464.9[化学工程—制药化工]