中国对虾养殖群体与野生群体线粒体控制区序列的比较  

Comparative analysis of the mitochondrial control region between the cultured and wild populations of Chinese shrimp (Fenneropenaeus chinensis)

作　　者：张辉[1] 高天翔[1] 庄志猛[2] 金显仕[2] 

机构地区：[1]中国海洋大学教育部海水养殖重点实验室,山东青岛266003 [2]中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《水产学报》2010年第8期1149-1155,共7页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2009AA09Z401);国家海洋局公益性科研专项(200905019)

摘　　要：比较分析了中国对虾养殖群体与野生群体mtDNA控制区序列。研究结果显示,在长度为563bp的mtDNA控制区片段中,中国对虾养殖群体与野生群体序列间存在一定程度的遗传差异。野生群体的基因多样度为0.9672,略高于养殖群体(0.9380),两群体间未检测到共享单倍型;野生群体mtDNA控制区核苷酸多样度为0.0106,养殖群体核苷酸多样度为0.0094,略低于野生群体的核苷酸多样度。基于K-2P模型计算得到中国对虾两群体间的平均遗传距离为0.0108,野生群体个体间平均遗传距离为0.0107,养殖群体个体间平均遗传距离为0.0095;单倍型最小跨度树和NJ系统树均未检测到显著的谱系结构。确切P检验显示两群体间没有随机交配现象(P=0.0009)。两群体间的FST值为0.0698(P=0.00),表明两群体间存在显著的遗传分化。In present study,genetic differentiation of the Chinese shrimp Fenneropenaeus chinensis between cultured（ 21 individuals） and wild populations（ 14 individuals,sequences were downloaded from the GenBank） was investigated using the sequence of mitochondrial control region （ CR ） .Four individuals of the F.merguiensis were chosen as the outgroup during the phylogenetic analysis in this study.Sequences were edited and aligned using DNA Star software and refined manually.Genetic diversity indices such as number of haplotypes,polymorphic sites,transitions,transversions,and indels were obtained using the program ARLEQUIN（ Ver.3.0） .Haplotype diversity（ h） and nucleotide diversity（ л） were calculated with DNASP 4.0.The nucleotide compositions and the average distances between populations and within population were calculated with MEGA 4.0.The Network software was used to draw the minimum spanning tree based on the haplotype data.The results were as follows：the A ＋ T content was higher than the G ＋ C content in both the populations,but the G ＋ C content was a little lower in the cultured population.The results indicated genetic divergence existed between the 2 populations based on the 563 bp sequences.The gene diversity of the wild population（ 0.967 2） was a little higher than the cultured population（ 0.938 0） .There was no shared haplotype between the 2 populations.The ratio of transitions/transversions of the wild populations and nucleotide diversity were 3.5 and 0.010 6 respectively while they were 4.0 and 0.009 4 for the cultured population.The nucleotide diversity of the wild population was a little higher than cultured population.The K-2P distance was 0.010 8 between the 2 populations,0.010 7 in the wild population and 0.009 5 in the cultured population.Both the NJ phylogenetic tree and the haplotype minimum spanning tree showed no significant genealogical structure.The exact test of population differentiation showed that the 2 populations were significantly different （ P=0

关 键 词：中国对虾 线粒体控制区 遗传多样性 养殖群体 野生群体 

分 类 号：Q178.1[生物学—水生生物学] S917[生物学—普通生物学]