大鼠中脑发育早期Nurr1时空模式及与TH表达的相关性  

The spatio-temporal pattern of Nurr1 in early development of rat midbrain,and the relationship with tyrosine hydroxylase expression

作　　者：董传明[1] 金国华[1] 谭雪锋[1] 田美玲[1] 秦建兵[1] 赵荷艳[1] 

机构地区：[1]南通大学人体解剖学教研室,神经生物学研究所,江苏省神经再生重点实验室,南通226001

出　　处：《神经解剖学杂志》2010年第5期513-516,共4页Chinese Journal of Neuroanatomy

基　　金：江苏省高校自然科学基金(09KJD310002)

摘　　要：目的:观察孤儿核受体相关因子1(Nuclear receptor-related factor1,Nurr1)在大鼠胚胎中脑发育过程中的时空表达模式及与酪氨酸羟化酶(tyrosine hydroxylase,TH)表达的相关性。方法:制备大鼠胚胎E12.5、E13.5、E14.5d中脑脑片,行Nurr1/TH的免疫荧光双标记。在荧光显微镜下分别计数每个视野下TH、Nurr1单标神经元和Nurr1/TH双标神经元的数量,用图像处理系统和Stata7.0软件对所得数据进行统计学分析。结果:Nurr1/TH双标神经元在E12.5d时较少,随着发育时间的推移逐渐增加,至E14.5d时TH阳性细胞中几乎均有Nurr1的表达。结论:本研究结果进一步从形态学的角度提示转录因子Nurr1在胚胎发育过程中对中脑多巴胺(dopamine,DA)能神经元的发育起重要作用。Objective：To investigate the spatio-temporal expression pattern of nuclear reaptor-related factor 1（Nurr1）during embryonic development of rat midbrain and it＇s relationship with tyrosine hydroxylase（TH）expression.Methods：Brain slices were obtained from E12.5,E13.5,E14.5 of rat midbrain.They were detected by immunohistochemical double labeling of Nurr1 and TH.The numbers of TH single-labeling neurons,Nurr1 single-labeling neurons and Nurr1/TH double-labeling neurons per field were counted respectively under a fluorescence microscope.Image processing system and Stata7.0 statistical software were used to analyze the data.Results：The present results showed that the numbers of Nurr1/TH double-labeling neurons were less on E12.5,which increased gradually with the procession of developmental time,and almost all the TH positive neurons were coexpressed with Nurr1 on E14.5.Conclusion：The morphological results indicated that Nurr1 may play an important role in the development of dopaminergic neurons.

关 键 词：核受体相关因子1 酪氨酸羟化酶 免疫荧光 中脑 大鼠 

分 类 号：R341[医药卫生—基础医学]