高压氧对大鼠颅脑爆震伤后皮层脑组织自噬与凋亡变化的影响  

Effect of hyperbaric oxygen on the changes of autophagy and apoptosis in cerebral cortex in rats with brain explosive injury

作　　者：汤宏 刘家传 张永明 杨艳艳 孙文江 张良潮[2] 李兵仓[2] 

机构地区：[1]解放军第一0五医院神经外科,合肥230031 [2]第三军医大学大坪医院野战外科研究所六室,重庆400042

出　　处：《中华神经医学杂志》2010年第10期1014-1017,共4页Chinese Journal of Neuromedicine

基　　金：南京军区十一五重点课题（06Z19）

摘　　要：目的观察高压氧治疗颅脑爆震伤后大鼠皮层脑组织自噬与凋亡表达的变化，探讨高压氧对脑细胞自噬和凋亡表达的影响及其意义。方法SD大鼠54只按照随机数字表法分为对照组（n=6）、爆震伤组（n=24）及爆震伤后高压氧治疗组（治疗组，n=24），对照组不进行致伤，爆震伤组和治疗组用600mgTNT电雷管在大鼠头部正上方垂直距离12cm处爆炸致伤，爆震伤组伤后不做任何治疗，治疗组分别于伤后3h、22h及以后每天同一时间行高压氧处理，爆震伤组和治疗组分别于6h、24h、3d、7d采用RT．PCR和Westernblotting检测皮层脑组织中Beclin-1和caspase-3的表达情况。结果与对照组相比，爆震伤组及治疗组各个时间点的Beclin-1和caspase-3表达均明显增加，差异有统计学意义（P〈0．05）；同一时间点上，治疗组中Beclin-1和caspase-3表达则较爆震伤组明显减少，差异有统计学意义（P〈0．05）。结论高压氧降低脑细胞的自噬和凋亡表达，此可能是其治疗颅脑损伤的机制之一。Objective To observe the changes of autophagy and apoptosis in the cerebral cortex in rats with brain explosive injury and received hyperbaric oxygen （HBO） treatment, and investigate the effect and significance of HBO on them. Methods Fifty-four SD rats were randomly divided into control group （n=6）, explosive injury group （n=24） and explosive injury treated with HBO group （n=24）. The control group was not injured and 600 mg TNT electric detonators were exploded over the brain of rats at a 12 cm distance in the explosive injury group and explosive injury treated with HBO group. HBO management was given to the explosive injury treated with HBO group 3 and 22 h after explosive injury and at the same time of the other 6 d. RT-PCR and Western blotting were used to detect the changes of Beclin-1 and caspase-3 in the cerebral cortex on the 6th and 24th, 3rd and 7th of injury. Results The expression levels of Beclin-1 and caspase-3 in the explosive injury group and explosive injury treated with HBO group were obviously higher than those in the control group （P〈0.05）. The levels of Beclin-1 and caspase-3 in the explosive injury treated with HBO group were significantly lower than those in the explosive injury group at the same time points （P〈0.05）. Conclusion The decreased expressions of autophagy and apoptosis in brain cells by HBO treatment might be one of the possible mechanisms of treating craniocerebral injury.

关 键 词：高压氧 细胞凋亡 自噬 

分 类 号：R686[医药卫生—骨科学]