骨髓间充质干细胞对烟雾吸入性损伤早期血管内皮细胞生长因子的影响  

Effects of bone marrow-derived mesenchymal stem cells engraftment on VEGF in lung tissue and in plasma of rabbits at early stage of smog inhalation injury

作　　者：朱峰[1] 郭光华[2] 陈雯[2] 王年云[2] 

机构地区：[1]南昌大学第一附属医院重症医学科,南昌330006 [2]南昌大学第一附属医院烧伤科,南昌330006

出　　处：《中华急诊医学杂志》2010年第9期938-941,共4页Chinese Journal of Emergency Medicine

基　　金：江西省教育厅青年科学基金项目（GJJ09432）

摘　　要：目的 探讨骨髓间充质干细胞（bone marrow-derived mesenchymal stem cells,MSCs）对烟雾吸入性损伤早期肺组织和血浆中血管内皮细胞生长因子（vascular endothelial cell growth factor,VEGF）以及血管外肺水的影响.方法 烟雾吸入性损伤模型达成后,64只兔随机（随机数字法）分成2组：致伤组（S组,n=32）和治疗组（M组,n=32）.S组伤后立即经耳缘静脉注入10 mL PBS液;M组伤后立即经耳缘静脉注入含兔第三代1×107个MSCs的PBS液10 mL.两组分别于伤前、伤后2 h,4 h和6h抽血后取兔肺组织,ELISA方法检测血浆和肺组织VEGF含量.伤后6 h另取肺组织作肺水质量分数测定.结果 致伤后肺组织VEGF在S组逐渐降低（P＜0.05）,而M组虽然也呈降低趋势（P＜0.05）,但与S组对应时间点相比却显著上升（P＜0.05）;血浆VEGF在S组逐渐升高（P＜0.05）,M组虽然也呈升高趋势（P＜0.05）,但与S组对应时间点相比却明显下降（P＜0.05）.实验6 h,M组肺水质量分数明显低于S组（P＜0.05）.结论 MSCs移植能升高早期烟雾吸入性损伤肺组织VEGF水平,降低外周血VEGF水平,减少血管外肺水,对烟雾吸入性损伤可能具有一定的保护作用.Objective To discuss the effects of bone marrow-derived mesenchymal stem cells （MSCs） on vascular endothelial growth factor （VEGF） in lung tissue and in plasma, and extra-vascular lung water at the early stage of smog inhalation injury. Method The rabbit model of smog inhalation injury was established by using home-made smog generator, and the rabbit models were randomly（random number） divided into control group （group S, n = 32） and MSCs treatment group （group M, n = 32）. Ten mL phosphate-buffered saline （PBS） was injected into ear marginal vein immediately after injury in rabbits of group S. The third generation of MSCs 1/10/10 mL PBS was injected into ear marginal vein immediately after injury in rabbits of group M. The levels of VEGF in peripheral blood and lung tissue were neasured 0 h,2 h,4 h and 6 hours after injection respectively, and analyzed. The right lung of rabbits was taken out to measure and calculate lung water mass fraction after experiment.Results In lung tissue, the levels of VEGF decreased gradually in group S （P ＜ 0.05）, and though the levels of VEGF in lung tissue appeared with significant decreasing trend in group M （P ＜ 0.05）, they were still higher than those of group S at corresponding intervals （ P ＜ 0.05）. In peripheral blood, the levels of VEGF increased gradually in group S （ P ＜ 0. 05）, and the levels of VEGF in group M appeared with markedly increasing trend （ P ＜ 0.05）,but they were lower significantly than those in group S at corresponding intervals （ P ＜ 0.05）. Conclusions MSCs engraftment to the rabbits with smog inhalation injury could increase VEGF in lung tissue, decrease VEGF in plasma and reduce extra-vascular lung water, showing protective effect on smog inhalation injury to a certain extent.

关 键 词：骨髓间充质干细胞 烟雾吸入性损伤 血管内皮细胞生长因子 血管外肺水 兔 

分 类 号：R644[医药卫生—外科学]