大鼠脑组织上清液诱导脂肪来源干细胞分化为神经细胞的实验研究  

Study on differentiation of adipose-derived stem cells into neural cells induced by supernatant liquid from brain tissue in rats

作　　者：王玮[1] 刘运海[1] 张宁[1] 杨兴东[2] 杨期东[1] 申向民[3] 杨靓[4] 

机构地区：[1]中南大学湘雅医院神经内科湖南省脑血管病防治中心,长沙410008 [2]北京海淀医院神经内科 [3]中南大学湘雅二医院神经内科 [4]中南大学湘雅医院神经外科,长沙410008

出　　处：《中华老年医学杂志》2010年第10期849-853,共5页Chinese Journal of Geriatrics

基　　金：湖南省卫生厅科研基金（A2004-002）

摘　　要：目的 观察正常大脑、脑梗死对侧及梗死侧大脑脑组织上清液对脂肪来源干细胞（ADSCs）向神经元样细胞分化的影响.方法 取SD大鼠腹膜后脂肪组织进行ADSCs分离培养;用正常脑组织上清液、脑组织梗死侧及对侧脑组织上清液对ADSCs进行诱导,免疫荧光法检测神经细胞标志物的表达,荧光显微镜下观察细胞阳性率.结果 （1）脑梗死侧组诱导组神经元特异性烯醇酶（NSE）、微管相关蛋白-2（MAP-2）、胶质纤维酸性蛋白（GFAP）表达率均高于其他组（P＜0.05）.（2）脑梗死对侧组及正常对照组诱导组NSE、MAP-2、GFAP表达率均高于未干预组（P＜0.05）.结论 脑梗死侧及脑梗死对侧组织上清液均能诱导大鼠ADSCs分化为神经元样细胞,表达神经细胞标志物,但前者作用更为明显.Objective To observe the effect of supernatant liquid from brain tissue on the differentiation of adipose-derived stem cells （ADSCs） into neural cells in normal brain tissues, the homogenate of infarcted cerebral hemisphere and the opposite side in the rats. Methods The ADSCs were obtained from rat retroperitoneal adipose tissue. The normal brain tissues, the homogenate of the infarcted cerebral hemisphere and thc opposite side got from middle cerebral artery occlusion in rats were used to induce ADSCs. Immunocytochemistry or immunofluorescence were used to identify the cell types at the 3rd day. Positive expression rate was counted by fluorescence microscope. Results （1）The neuron-specific enolase （NSE） positive cells, microtubule-associated protein 2 （MAP-2） positive cells and glial fibrillary acidic protein （GFAP） positive cells were much more in the homogenate of the infracted cerebral hemisphere than in others （P＜0.05）. （2）The NSE positive cells, MAP-2 positive cells and GFAP positive cells were much more in the homogenate of the normal brain tissues and the opposite side than normal level （ P ＜ 0. 05 ） . Conclusions The homogenate of the infracted cerebral hemisphere and the opposite side can induce adipose-derived stem cells into neural-like cells and express neural cells markers in rats.

关 键 词：干细胞 细胞分化 细胞 培养的 脑缺血 

分 类 号：R686[医药卫生—骨科学]