低剂量氯胺酮对食蟹猴行为、海马和前额叶皮质超微结构的影响  

Effects of low-dose ketamine administration on behavior and ultrastructures of the hippocampus and prefrontal cortex in Cynomolgus monkeys

作　　者：王力[1] 江虹[1] 于洪鸾[2] 孙琳[1] 潘芳[1] 

机构地区：[1]山东大学医学院心理研究所,济南250012 [2]山东大学齐鲁医院心理科,济南250012

出　　处：《山东大学学报（医学版）》2010年第9期64-67,71,共5页Journal of Shandong University:Health Sciences

基　　金：香港特别行政区政府禁毒署资助项目(0800-48)

摘　　要：目的研究新型毒品氯胺酮长期低剂量应用对食蟹猴行为学、海马和前额皮质超微结构的影响。方法 12只食蟹猴随机分为氯胺酮组(8只)和对照组(4只)。氯胺酮组每日静脉注射氯胺酮(1mg/kg),对照组每日静脉注射生理盐水(1mL/kg),定期对12只猴进行行为学分析,6个月后处死取材,透射电镜下观察海马及前额皮质超微结构的变化。结果与对照组相比,氯胺酮组运动行为无统计学意义(P﹥0.05);海马区出现神经细胞坏死,胶质细胞凋亡及大量脂褐素堆积;前额皮质区呈现大量自噬体,粗面内质网数量明显减少,神经元坏死和胶质细胞凋亡,而对照组两区域电镜超微结构正常。结论氯胺酮低剂量长期应用可导致海马及前额皮质超微结构的变化,主要表现为神经细胞的变性、坏死和凋亡,这一变化较行为学变化更为敏感。Objective To explore effects of long-term low-dose ketamine on behavior and ultrastructures of the hippocam-pus and prefrontal cortex in Cynomolgus monkeys.Methods 12 male Cynomolgus monkeys were randomly divided into the ketamine group （n=8） and the control group （n=4） .The ketamine group was daily intravenously injected with ket-amine（1 mg/kg） ,while the control group injected with normal saline（1 mL/kg） .Observations on behavior were regularly made and ultrastructural changes of the hippocampus and prefrontal cortex were observed by a transmission electron micro-scope after 6 months.Results There was no statistically significant difference in locomotor activities between the two groups（P﹥0.05） .Ultrastructural changes in the ketamine group,including neuron necrosis,glial cell apoptosis and li-pofuscin accumulation,appeared in the hippocampus; a mass of autophagosomes,reduction of the rough endoplasmic reticulum,neuron necrosis,and glial cell apoptosis were observed in the prefrontal cortex in the ketamine group,while not observed in the control group.Conclusion Long-term low-dose ketamine induces ultra-structural changes in the hippo-campus and prefrontal cortex,including neuron degeneration,necrosis and apoptosis,which appear earlier than behavioral changes do.

关 键 词：氯胺酮 食蟹猴 行为学 超微结构 细胞死亡 

分 类 号：R395.1[哲学宗教—心理学]