不同浓度骨髓基质细胞修复大鼠牙周组织缺损的实验研究  

Effects of Bone Marrow Stromal Cells with Different Seeding Densities on Periodontal Defect Repair in Sprague Dawley Rats

作　　者：李艳芬[1] 赵欣[1] 闫福华[1] 骆凯[1] 郭建斌[1] 江俊[1] 

机构地区：[1]福建医科大学附属口腔医院,福州350002

出　　处：《福建医科大学学报》2010年第4期249-252,共4页Journal of Fujian Medical University

基　　金：国家自然科学基金(30471892);福建省自然科学基金(2008J0085)

摘　　要：目的探讨不同浓度骨髓基质细胞(BMSCs)对牙周组织再生的影响。方法将BMSCs以不同浓度(5×105,5×106,5×107mL-1)与胶原膜BME-10X复合培养后,复合物和空白胶原膜BME-10X分别植入SD大鼠牙周缺损部位,并覆盖膨体聚四氟乙烯膜(e-PTFE膜),以缺损处只覆盖e-PTFE膜为对照组,4周后取材,H-E染色观察牙周组织再生情况。结果各实验组新生牙槽骨面积与对照组相比有明显增加(P<0.05),5×106,5×107mL-1组的新生牙槽骨量与5×105mL-1和空白BME-10X组比较,差别有统计学意义(P<0.05),而5×106,5×107,5×105mL-1与空白BME-10X组组间比较则无统计学意义(P>0.05);各组新生牙骨质面积之间差别无统计学意义(P>0.05)。结论 BMSCs浓度可影响其体内成骨能力,高浓度BMSCs能有效促进牙周组织再生。Objective To evaluate the effects of bone marrow stromal cells（BMSCs） with different densities on periodontal regeneration.Methods BMSCs were seeded on the collagen membranes with different concentrations（5×105 mL-1,5×10 mL-1,and 5×107 mL-1）.The complex and collagen membranes without cells were randomly implanted into the periodontal defects in Sprague Dawley rats and covered with e-PTFE membranes.The defects with E-PTFE membrane alone served as a control.Periodontal regeneration was evaluated by histometric measurements with HE staining at 4 weeks after transplantation.Results Compared with that of the control group,the area of newly formed alveolar bone was significantly increased in all experimental groups（P0.05）.The area of newly formed alveolar bone of the 5×106 mL-1 and 5×107 mL-1 groups was significantly larger than that of the 5×105mL-1 and blank groups,while new bone formation was not significantly different between the 5×106 mL-1 and 5×107 mL-1 groups,the 5×105 mL-1 and blank groups.There was no significant difference in the area of newly formed cementum.Conclusion BMSCs with different densities can affect the osteogenic capacity in vivo.BMSCs at high concentration can effectively promote the periodontal tissue regeneration.

关 键 词：组织工程 引导组织再生 牙周 牙周组织 骨髓细胞 胶原 疾病模型 动物 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R318[医药卫生—基础医学]