bFGF基因转染的牙龈成纤维细胞与脱细胞真皮基质复合物的体外构建  

Construction of Acellular Dermal Matrix Compounded with hbFGF Gene-Modified Gingival Fibroblasts

作　　者：刘崇武[1] 陈欣戬[1] 李艳芬[1] 闫福华[1] 

机构地区：[1]福建医科大学附属口腔医院牙周科,福州350002

出　　处：《福建医科大学学报》2010年第4期257-260,共4页Journal of Fujian Medical University

基　　金：福建医科大学科学研究发展基金(XZ04011);福建医科大学附属口腔医院重点学科建设学术发展基金(闽医大口腔[2008]39号)

摘　　要：目的研究碱性成纤维细胞生长因子(bFGF)基因修饰的牙龈成纤维细胞(GFs)与组织工程支架材料脱细胞真皮基质(ADM)复合后的生长、结合情况,为基因治疗与组织工程联合应用于牙周组织缺损再生治疗提供依据。方法将转染bFGF的GFs接种于ADM真皮面,通过荧光显微镜、激光共聚焦扫描显微镜、透射电镜及组织学检查,观察细胞的生长状况及其与支架材料的复合情况。结果基因修饰的牙龈成纤维细胞复合ADM膜后生长良好,24 h细胞已贴附、伸展。透射电镜可见细胞在支架材料中生长,并与支架材料结合紧密。结论转染bFGF基因的的GFs与ADM膜复合物有望应用于牙周组织工程。Objective To study the growth of human basic fibroblast growth factor（hbFGF） gene-modified gingival fibroblasts（GFs） implanted on the acellular dermal matrix（ADM） and provide evidence for gene therapy combined with tissue engineering for the regeneration of periodontal tissue defect.Methods hbFGF gene-transfected GFs were implanted onto ADM.The formation of cell-scaffold composition was observed by fluorescence microscopy,confocal laser scanning microscopy,transmission electron microscopy,hematoxylin and eosin stain.Results The transfected cells grew well on the ADM.Cell attachment and spreading were observed 24 h after implantation.Transmission electron microscopy showed that cells grew well and closely integrated with the scaffold materials.Conclusion hbFGF gene-modified GFs-ADM compound can be used in the periodontal tissue engineering.

关 键 词：成纤维细胞生长因子2 牙周组织 牙龈 成纤维细胞 基因疗法 转染 组织工程 细胞外基质 真皮 

分 类 号：R322.41[医药卫生—人体解剖和组织胚胎学] R392.12[医药卫生—基础医学]