幽门螺杆菌相关性胃十二指肠疾病患儿MMP-7基因多态性研究  

Analysis of MMP-7 gene polymorphisms in upper gastrointestinal disease infected with Helicobacter pylori in children

作　　者：单庆文[1] 经承学[1] 王琳琳[1] 吕自力[2] 唐清[1] 云翔[1] 连淑君[1] 

机构地区：[1]广西医科大学第一附属医院儿科,广西南宁530021 [2]广西医科大学第一附属医院病理科,广西南宁530021

出　　处：《临床儿科杂志》2010年第10期916-921,共6页Journal of Clinical Pediatrics

基　　金：广西科学基金资助项目(No.桂科青0991030)

摘　　要：目的研究基质金属蛋白酶-7(MMP-7)基因启动子多态性与儿童幽门螺杆菌(H.pylori)相关性慢性胃炎、十二指肠溃疡的易感性和临床特征的关系。方法提取100例慢性胃炎、32例十二指肠溃疡和102例健康对照组儿童的外周血或胃黏膜基因组DNA,采用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP)和测序方法检测其MMP-7基因-181A/G多态性位点的基因型,分析该基因的基因型和等位基因频率在病例组和对照组人群中的分布及基因型分布与临床病理特征的关系,采用反转录-聚合酶链反应(RT-PCR)方法检测各组胃黏膜MMP-7 mRNA的表达。结果 MMP-7基因-181A/G多态性位点的基因型和等位基因频率在病例组和对照组人群中的分布差别无统计学意义,亦与H.pylori易感性无关。MMP-7基因-181A/G多态性不影响MMP-7 mRNA在胃黏膜中的表达,且与胃窦黏膜慢性炎症程度无相关性。结论 MMP-7基因-181A/G多态性与儿童慢性胃炎和十二指肠溃疡发生的易感性无关。Objective To investigate the relationship between the promoter of matrix metalloproteinase-7 （MMP-7） gene polymorphisms to and the susceptibility and clinical features of chronic gastritis and duodenal ulcer with or without H. pylori infection in children. Methods Genomic DNA was obtained from peripheral blood or gastric biopsies in 100 pediatric patients with chronic gastritis and 32 pediatric patients with duodenal ulcer and 102 healthy children. The promoter of MMP-7-181A/G gene polymorphisms were genotyped by polymerase chain reaction restriction fragment length polymorphism （PCR-RFLP） and sequencing. The genotype distributions and allele frequencies were compared between pediatric patients and controls, and the association of genotypes with clinic pathological features was analyzed. MMP-7 mRNA expression level in gastric mucosa was determined by reverse transcription polymerase chain reaction biopsy-based tests. Results The genotype distributions and allele frequencies of MMP-7-181A/G gene polymorphisms were similar in patients and healthy subjects. The MMP-7-181A/G gene polymorphisms were not associated with H.pylori status. MMP-7-181A/G gene polymorphisms did not affect MMP-7 mRNA expression level and not associated with the degree of antrum chronic inflammation. Conclusions MMP-7-181A/G gene polymorphisms are not associated with susceptibility to chronic gastritis and duodenal ulcer in children.

关 键 词：基质金属蛋白酶-7 基因多态性 幽门螺杆菌 儿童 胃炎 十二指肠溃疡 

分 类 号：R725.7[医药卫生—儿科]