金刚烷胺和奥司他韦体外抗H5N1亚型禽流感病毒的作用  

Antiviral Medication of Highly Pathogenic A(H5N1) Avian Influenza Viruses to Amantadine and Oseltamivir In Vitro

作　　者：刘丽华[1] 朱翔[1] 张宇锋[1] 温景芸[2] 张永标[3] 冼盈[1] 林潮双[1] 廖明[4] 辛朝安[4] 张扣兴[1] 

机构地区：[1]中山大学附属第三医院感染科,广东广州510630 [2]中山大学附属第三医院肿瘤科,广东广州510630 [3]中山大学附属第三医院急诊科,广东广州510630 [4]华南农业大学,广东广州510642

出　　处：《中山大学学报（医学科学版）》2010年第5期635-640,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省自然科学基金(06034508);广东省医学科研基金(A2006212);广东省科技计划项目(2008B030301302)

摘　　要：【目的】观察体外单用金刚烷胺、奥司他韦及两药联合使用对两株H5N1亚型的禽流感病毒分离株(AIV127、AIV174)的抑制作用,了解广东地区分离的不同H5N1毒株之间药物敏感性的差异,为临床合理应用抗病毒药物提供科学依据。【方法】建立体外H5N1感染模型,根据药物与病毒、细胞的不同作用,通过3种给药方式,使用细胞病变(CPE)、组织培养半数感染量(TCID50)评估抗病毒药物对H5N1的体外抑制作用。【结果】三种给药方式金刚烷胺联合奥司他韦,均比单用金刚烷胺体外抑制AIV127及AIV174效果明显(P<0.01);而仅在治疗给药方式,两者联合比单用奥司他韦效果明显(P<0.01)。三种给药方式金刚烷胺、奥司他韦及联合用药对同一亚型H5N1AIV127比AIV174毒株均较敏感(P<0.01)。【结论】抗病毒药物对不同的禽流感病毒毒株之间敏感性存在差异,应根据不同毒株给予相应的抗病毒治疗。Objective To observe inhibition of Amantadine,Oseltamivir and double combination on H5N1 in vitro and further investigate antiviral activity of drugs against H5N1 isolated locally.Methods Cytopathic effect（CPE）,hemagglutinin（HA） titers and TCID50 of virus were applied to observe the antiviral activity of H5N1（AIV 174 and AIV 127） with three different medications,including treated,prophylactic medications and drugs affected H5N1 directly.Results The inhibition of conbination is better than amantadine alone for H5N1（AIV 174 and AIV 127） with three medications（P0.01） whereas the combination is better than Oseltamivir only through treated medication（P0.01）.Inhibition of Amantadine,Oseltamivir alone and double combination for AIV127 was more sensitive to AIV174 through three medications（P0.01）.Conclusions Sensitivity of antiviral drugs differs from different avian influenza strains,corresponding antiviral treatments should be given according to different viral strains locally in clinic.

关 键 词：药物 禽流感病毒 H5N1亚型 抗病毒活性 

分 类 号：R563.1[医药卫生—呼吸系统]