Muscarinic receptor-mediated calcium changes in a rat model of facial nerve nucleus injury  

作　　者：Dawei Sun Huamin Liu Fugao Zhu Yanqing Wang Junfeng Wen Rui Zhou Yanjun Wang Banghua Liu 

机构地区：[1]Department of Otolaryngology-Head and Neck Surgery, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China [2]Department of Oncology, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province, China [3]Department of Science and Education, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, Shandong Province China [4]School of Public Health, Shandong University, Jinan 250012, Shandong Province, China [5]Department of Otolaryngology-Head and Neck Surgery, Union Hospital of Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China

出　　处：《Neural Regeneration Research》2010年第18期1418-1422,共5页中国神经再生研究（英文版）

基　　金：Youth Scientific Research Foundation of Qingdao University (2007)

摘　　要：The muscarinic receptor modulates intracellular free calcium ion levels in the facial nerve nucleus via different channels. In the present study, muscarinic receptor-mediated free calcium ions levels were detected by confocal laser microscopy in the facial nerve nucleus following facial nerve injury in rats. There was no significant difference in muscarinic receptor expression at the affected facial nerve nucleus compared with expression prior to injury, but muscarinic receptor-mediated free calcium ion levels increased in the affected side following facial nerve injury （P 〈 0.01）. At day 30 after facial nerve injury, 50 pmol/L muscarinic-mediated free calcium ion levels were significantly inhibited at the affected facial nerve nucleus in calcium-free artificial cerebrospinal fluid, and the change range was 82% of artificial cerebrospinal fluid （P 〈 0.05）. These results suggest that increased free calcium ion concentrations are achieved by intracellular calcium ion release, and that the transmembrane flow of calcium ions is also involved in this process.The muscarinic receptor modulates intracellular free calcium ion levels in the facial nerve nucleus via different channels. In the present study, muscarinic receptor-mediated free calcium ions levels were detected by confocal laser microscopy in the facial nerve nucleus following facial nerve injury in rats. There was no significant difference in muscarinic receptor expression at the affected facial nerve nucleus compared with expression prior to injury, but muscarinic receptor-mediated free calcium ion levels increased in the affected side following facial nerve injury （P 〈 0.01）. At day 30 after facial nerve injury, 50 pmol/L muscarinic-mediated free calcium ion levels were significantly inhibited at the affected facial nerve nucleus in calcium-free artificial cerebrospinal fluid, and the change range was 82% of artificial cerebrospinal fluid （P 〈 0.05）. These results suggest that increased free calcium ion concentrations are achieved by intracellular calcium ion release, and that the transmembrane flow of calcium ions is also involved in this process.

关 键 词：facial nerve injury muscarinic receptor facial nerve nucleus calcium ion THAPSIGARGIN 

分 类 号：Q463[生物学—生理学] Q425