甲烷利用克雷伯氏菌ME17菌株培养参数优化研究  

Optimization of Liquid Fermentation Condition for Methane Utilizing Klebsiella sp. ME17

作　　者：赵翠翠[1] 郑军[1] 赵艮贵[1] 

机构地区：[1]山西大学生物技术研究所化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《山西大学学报（自然科学版）》2010年第3期453-457,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金重点项目(50534100)

摘　　要：利用响应面分析法对克雷伯氏菌菌株ME17的液体培养条件进行了优化,研究了温度、接种量、甲烷含量和培养基pH对菌株ME17生长的影响.在单因素实验的基础上,利用Box-Benhnken设计和响应面分析法对培养温度、接种量和甲烷含量进行分析,得到最佳培养条件为:pH6.0,培养温度24.4℃,接种量6.7%,甲烷含量25%.在优化培养条件下,细菌生物量约是初始培养条件下的1.7倍,延滞期缩短45h.该菌株初步应用于甲烷气体的脱除,脱除率达84.7%,表明该菌株在优化培养条件下能良好地脱除空气中的甲烷气体.The optimization of liquid fermentation condition for Klebsiella sp.ME17 was analyzed by response surface method,and the effects of temperature,inoculum volume,methane content and initial pH of media on the cell growth were studied.Based on the single factor experiments,the temperature,inoculum volume and methane content were analyzed by Box-Benhnken design and response surface method.The optimum conditions were as follows：pH 6.0,temperature at 24.4 ℃,inoculum volume 6.7% and methane content 25%.Under the optimal culture conditions,the ME17 production was increased to 1.7 times and its log phase was reduced for 45 h than preliminary culture.The removal of methane gas by ME17 was preliminary investigated and the removal rate of methane gas reached 84.7%.The results indicated that the strain which was acclimated on the optimization of liquid fermentation condition was capable of removing of methane.

关 键 词：克雷伯氏菌 响应面法 甲烷脱除率 

分 类 号：Q93[生物学—微生物学]