不同抗性玉米接种矮花叶病毒后的生理生化变化研究  

Physiological and Biochemical Changes of Different Resistant Corn Infected with Maize Dwarf Mosaic Virus

作　　者：闫慧娟[1] 韩玉杰[1] 周小梅[1] 张彦芹[2] 张明义[2] 

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]山西省农业科学院旱地农业中心,山西太原030031

出　　处：《山西大学学报（自然科学版）》2010年第3期458-462,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省自然科学基金(2007011080)

摘　　要：选取对玉米矮花叶病毒的抗感病品种沈137和Mo17,以未接种植株为对照,接种矮花叶病毒后,对144h内叶片中的可溶性糖、丙二醛和过氧化氢含量及防御反应酶系中的过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)和苯丙氨酸解氨酶(PAL)活性变化进行了研究.试验结果显示,接病后抗病品种的可溶性糖含量变化不大或有上升而感病品种则下降;丙二醛含量在抗感玉米中均变化不大;抗病玉米中H2O2含量下降,而感病玉米中无太大变化.接病后的玉米叶片中4种酶活性的变化表现为抗病品种的CAT活性呈现出先降低后升高,感病品种变化不大;抗病品种的POD活性高于对照,感病品种则以下降为主;SOD活性在感病玉米中呈升高,抗病品种中变化不大;PAL酶活的变化是抗病品种表现为先升高随后下降,而感病玉米则相反.用干燥和冷冻保存后的病叶接病,玉米的发病情况是冷冻保存的叶片比干燥保存的叶片发病率高.Dynamics in soluble sugar,MDA,H2O2 content and the activities of CAT,POD,SOD,PAL in two varieties of corn（Shen 137 and Mo17） leaves were studied for 144 hours after inoculation,which were contrasted with those of noninoculation.The results showed that the soluble sugar in resistant variety mainly manifest little change or rise and that in the susceptible variety decrease.The MDA had no significant changes both in resistant and susceptible varieties.The H2O2 content decreased in the resistant variety and had no change in the susceptible variety.The CAT activity started to decreased then increased in the resistant variety,and it was similar to control in the susceptible variety.The POD activity was higher than the control in the resistant variety and mainly lower than in the susceptible variety.The SOD activity increased in the susceptible variety and was same as the control in the resistant variety.The PAL activity began to increase then decreased in the resistant variety,while that was the opposite in the susceptible variety.The incidence of maize was lower after inoculated with diseased leaves conserved in dry condition than that in frozen condition.

关 键 词：玉米 矮花叶病毒 生理生化 

分 类 号：Q945.8[农业科学—植物病理学]