高效液相色谱法测定蟾酥药材中脂蟾毒配基和华蟾酥毒基的含量  

Determination of recibufogen and cinobufagin in Venenum Bufonis by HPLC

作　　者：董玮玮[1,2] 周伟[2] 李顺旭[2] 彭江丽[2] 杨大坚[2] 

机构地区：[1]上海交通大学药学院,上海200240 [2]深圳市中药药学及分子药理学研究重点实验室暨香港理工大学现代中药研究所,广东深圳518057

出　　处：《中南药学》2010年第10期721-724,共4页Central South Pharmacy

基　　金：香港赛马会慈善信托基金(编号:JCICM-5-07)

摘　　要：目的建立蟾酥药材中脂蟾毒配基和华蟾酥毒基的含量测定方法 ,并对不同品质的药材中2种成分进行含量测定。方法采用高效液相色谱法,色谱柱：Phenomenex C18柱（4.6 mm×250 mm,5μm）;流动相：乙腈-0.3%乙酸溶液,梯度洗脱,流速：0.7 mL.min-1;检测波长：296 nm;柱温：30℃。结果脂蟾毒配基在0.032 52.080μg与峰面积线性关系良好（r=0.999 7）,回收率为99.6%,RSD=2.0%;华蟾酥毒基在0.031 92.040μg与峰面积线性关系良好（r=0.999 6）,回收率为102.5%,RSD=2.0%。结论该法可靠、准确、简便,可作为蟾酥药材的质量控制方法 。Objective To establish a method for the determination of the content of cinobufagin and resibufogenin in Venenum Bufonis.Methods Phenomenex C18（4.6 mm × 250 mm,5 μm） column was used.The mobile phase consisted of acetonitrile and water（0.3% acetic acid） with gradient elution.The detection wavelength was 296 nm,the flow rate was 0.7 mL·min-1,and the column temperature was 30 ℃.Results The resibufogenin content showed a good linearity within 0.032 5-2.080 μg（r=0.999 7）.The average recovery was 99.6% and RSD was 2.0%（n=6）.The cinobufagin content showed a good linearity within 0.031 9-2.040 μg（r=0.999 6）.The average recovery was 102.5% and RSD was 2.0%（n=6）.Conclusion The method is simple and reliable,which can be used for the quality control of Venenum Bufonis.

关 键 词：蟾酥 脂蟾毒配基 华蟾酥毒基 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学] R284.2[医药卫生—药学]