本地毛形线虫49 ku排泄分泌蛋白基因的克隆及序列分析  

Cloning and Sequencing Analysis of 49 ku ES Protein Gene for Trichinella Nativa

作　　者：郑宝亮[1] 边传周[1] 王秀荣[2] 宋铭忻[3] 

机构地区：[1]郑州牧业工程高等专科学校,河南郑州450011 [2]中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室兽医生物技术国家重点实验室,黑龙江哈尔滨150001 [3]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《动物医学进展》2010年第10期68-71,共4页Progress In Veterinary Medicine

摘　　要：为了获得本地毛形线虫(Trichinella nativa)49 ku ES蛋白的结构基因,用Trizol提取T.nativa肌幼虫的总RNA,用RT-PCR方法扩增出了编码T.nativa49 ku排泄分泌蛋白的结构基因。将PCR产物与T载体连接并经过大肠埃希菌增殖后送检测序。序列测定表明,目的基因TNPG长度为951bp,核苷酸序列同已发表的T.spiralis相应的序列P49同源性为98.63%,所推导的氨基酸序列同源性为99.05%。为T.nativa49 ku ES蛋白结构基因的进一步研究打下基础。The target gene of 49 ku ES protein of T.nativa was searched here.The total RNA of Trichinella nativa was isolated using Trizol after the worms being pestled.The gene of the worm which encoding 49 ku ES protein was amplified by RT-PCR.PCR products were sequenced after being linked with T-vector and propagated by E.coli.It was shown from the sequence analysis that the target gene TNPG was consisted of 951 bp and 98.63% nucleotide homology to the sequence of P49 from T.spiralis,and 99.05% homology of deduced amino acid between TNPG and P49.This study lays a base for the more research on the 49ku ES protein of T.nativa.

关 键 词：本地毛形线虫 旋毛形线虫 49ku排泄分泌蛋白 基因序列 

分 类 号：S855.9[农业科学—临床兽医学]