盐芥ICE1转录因子的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of a Transcription Factor ICE1 from Thellungiella halophila

作　　者：轩春雷[1] 肖向文[2] 李晓波[2] 祝建波[1] 

机构地区：[1]石河子大学生命科学学院,石河子832003 [2]中国科学院新疆理化技术研究所,乌鲁木齐830023

出　　处：《生物技术通报》2010年第11期108-114,共7页Biotechnology Bulletin

基　　金：国家转基因重大专项(2008ZX005-004);国家"863"重点项目(2007AA021401)

摘　　要：ICE1基因编码一个MYB类型的碱性螺旋-环-螺旋(bHLH)转录因子,在冷胁迫条件下调节CBF基因的表达,能够提高植物抗寒性。利用盐芥5′EST序列和拟南芥ICE13′UTR保守序列设计引物,从盐芥基因组中克隆得到了1个2525bp的基因xhICE。生物信息学分析,表明该基因具有4个外显子,4个内含子,外显子/内含子边界符合经典的GT-AG规则。由此推导的cDNA包含一个1500bp的开放阅读框,编码500个氨基酸。与拟南芥ICE1相比,二者的内含子具有相同的类型和相对保守的位置,在核酸水平与氨基酸水平高度同源,并且具有相同的bHLH结构域。以上分析都表明,xhICE基因可能是盐芥ICE1基因,涉及抗寒相关的CBF转录调控途径。ICE1 encodes a MYC-type basic helix-loop-helix transcription factor that regulates the transcription of CBF genes under cold stress,which can improve the chilling tolerance of plants.In this study,we make use of the 5＇EST sequence of Thellungiella halophila and the 3＇ UTR sequence of Arabidopsis thaliana ICE1 to design primer and clone the ICE1 gene from Thellungiella halophila.The bioinformatics analysis showed that this gene consists of four exons and four introns,and the intron-exon boundaries conform to the＂GT-AG＂rule.It follows that this predicted cDNA has an open reading frame（ORF）of 1 500 bp in length,encoding a protein of 500 amino acids.In comparison to the genome sequence of Arabidopsis thaliana ICE1,both introns have the same type and relatively conservative position,highly homogeneity at amino acid and nucleic acid level and have a potential basic helix-loop-helix.All results indicate that xhICE gene might be Thellungiella halophila ICE1 gene,that can be involved in the CBF transcriptional regulatory paths.

关 键 词：转录因子 表达序列标签 生物信息学 ICE1 

分 类 号：Q943.2[生物学—植物学]