单核细胞增生李斯特菌毒力基因inlB/actA双缺失突变株的构建  

Construction of a Mutant Strain of Listeria monocytogenes with a Deletion of inlB and actA

作　　者：王莉[1] 冯飞飞[1] 张强[1] 冯莹颖[1] 邓灵福[1] 张晓莉[1] 罗勤[1] 

机构地区：[1]华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,武汉430079

出　　处：《生物技术通报》2010年第11期182-185,共4页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30970111);湖北杰出青年基金项目(2009CDA124);华中师范大学科研项目(CCNU09Y01001);华中师范大学研究生自主科研项目(2009028)

摘　　要：单核细胞增生李斯特菌inlB和actA毒力基因的编码产物InlB和ActA是与其致病性相关的重要毒力因子,与该菌在细胞间扩散、传播有着直接的关系,其中肌动蛋白聚集因子ActA是细菌由细胞浆扩散至相邻细胞所必须的因子,而内化素InlB在对肝细胞的侵袭过程中起着重要作用。本研究中利用同源重组技术在inlB缺失菌株基础上成功构建了毒力基因inlB和actA双缺失的突变株,获得减毒突变株,为构建预防人类和动物疾病的疫苗载体奠定了基础。ActA and InlB,encoded by actA and inlB,are essential virulence factors of Listeria monocytogenes（LM）.The virulence of LM is directly related to its ability to spread from cell to cell without leaving the intracellular milieu.Among the bacterial factors involved in cell-to-cell spread,actin-polymerizing protein ActA is required for bacterial spread to adjacent cells,while the internalin B（InlB）plays an important role in the process of invasion the liver cells.Based on homologous recombination technology,we constructed a double gene-mutant strain by deleting two virulence genes,inlB and actA.The mutant strain could be further studied as a vaccine candidate to prevent listeriosis and used as a vector to deliver heterologous antigens.

关 键 词：单核细胞增生李斯特菌 基因敲除 inlB基因 actA基因 同源重组 

分 类 号：Q78[生物学—分子生物学]