柔嫩艾美耳球虫野外分离株对4种抗球虫药的耐药性检测  

Determination of the different effects of four anticoccidia drugs against field strains of Eimeria tenella

作　　者：王凤[1] 曾明华[1] 吴金凤[1] 汪金菊[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036

出　　处：《安徽农业大学学报》2010年第4期631-634,共4页Journal of Anhui Agricultural University

基　　金：安徽省高等学校省级自然科学研究项目(KJ2009A034Z)资助

摘　　要：该试验采集安庆、肥东及巢湖3个地区的3株盲肠球虫,进行柔嫩艾美耳球虫(E.tellena)单卵囊的分离,采用鸡体实验法对癸氧喹酯、尼卡巴嗪、球痢灵和磺胺氯吡嗪钠进行耐药性检测,以抗球虫指数(ACI)、病变记分减少率(RLS)、相对盲肠卵囊产量(ROP)和最适抗球虫活性百分率(POAA)4项指标综合判定。结果表明,安庆株、肥东株及巢湖株均对癸氧喹酯敏感,对尼卡巴嗪均完全产生耐药;安庆株对球痢灵中度耐药,对磺胺氯吡嗪钠重度耐药;肥东株对球痢灵轻度耐药,对磺胺氯吡嗪钠重度耐药;巢湖株对球痢灵重度耐药,对磺胺氯吡嗪钠完全耐药。In this experiment, three Eimeria tenella strains were isolated and purified from Anqing,Feidong, and Chaohu broiler farms,then the Body-testing method was used to test the drug resistance of the three strains to four anticoccidials：Decoquinate, Nicarbazin and Zoalene and Sodium sulfa chlorpromazine. The criteria used for evaluation the resistance included anticoccidianl index （ ACI）, reduction of lesion scores （ RLS）, percent of optimum anticoccidial activity（POAA） and relative at oocyst production （ROP）. The results showed that Anqing strain, Feidong strain and Chaohu strain all assumed the negative to Decoquinate, but have a complete resistance to Nicarbazin. Anqing strain has a moderate resistance to Zoalene, and a serious resistance to Sodium sulfa chlorpromazine. Feidong strain has a light resistance to Zoalene, and a severe resistance to Sodium sulfa chlorpromazine. Chaohu strain has a severe resistance to Zoalene, and a complete resistance to Sodium sulfa chlorpromazine.

关 键 词：柔嫩艾美耳球虫 鸡体实验 抗球虫药 耐药性 

分 类 号：S852.723[农业科学—基础兽医学]