酚-硫酸法测定木素磺酸铵中糖含量的研究  

Saccharide Content Determination in Ammonium Lignin Sulphonate with Phenol-sulfuric Acid Method

作　　者：胡建鹏[1] 王勇[1] 郭明辉[1] 

机构地区：[1]东北林业大学生物质材料与技术教育部重点实验室,黑龙江哈尔滨150040

出　　处：《西南林学院学报》2010年第5期65-67,71,共4页Journal of Southwest Forestry College

基　　金：中央高校基本科研业务费专项资金项目(DL09EB01-1)资助;黑龙江省重点自然科学基金项目(ZD200808-01)资助;黑龙江省重点科技攻关项目(GA09B201-07)资助;哈尔滨市重点科技攻关项目(GJ2007GG002235)资助

摘　　要：采用酚-硫酸法测定木素磺酸铵中糖含量,以葡萄糖为对照,苯酚-硫酸作显色剂,用紫外分光光度计在490 nm处测定吸光度。结果显示:葡萄糖浓度为20～120μg/mL时线性关系显著,R2=0.995 9;精密度RSD=0.034%,稳定性RSD=0.102%,回收率98.99%,RSD=0.871%,重现性RSD=0.336%。酚-硫酸法测定木素磺酸铵中糖含量简便易行,精密度、稳定性、回收性、重现性良好。Taking glucose as CK and phenol sulfuric acid as chromogenic reagents,the saccharide content in ammonium lignin sulphonate was determined by means of phenol sulfuric method with UV spectrophotometer in 490 nm section.The results showed that there was a significant linear correlation between the glucose content and the photometric value when the glucose concentration was in the range of 20～120 μg/mL,the coefficient R2 was 0.995 9,with precision RSD as 0.034%,stability RSD as 0.102%,recovery rate as 98.99%,recovery RSD as 0.871%,and the repetition RSD as 0.336%.It was indicated that the phenol sulfuric acid method for determining saccharide content in ammonium lignin sulphonate was a way characterized with simplicity,practicality,high precision,good stability and repetition with high recovery rate.

关 键 词：酚-硫酸法 标准曲线 木素磺酸铵 糖含量 

分 类 号：TS743[轻工技术与工程—制浆造纸工程] Q530.3[生物学—生物化学]