离子束介导的水稻早熟变异株系AFLP分析  

AFLP ANALYSIS OF EARLY MATURING RICE MUTANTS INDUCED BY ION BEAN MEDIATED

作　　者：姬生栋[1] 王知丰[1] 王聪睿[2] 岳春晖[1] 陈鹏[1] 朱德来[1] 王海莎[1] 侯磊磊[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]新乡医学院,河南新乡453003

出　　处：《核农学报》2010年第5期895-900,共6页Journal of Nuclear Agricultural Sciences

基　　金：河南省自然科学基金(092300410082);河南师范大学应用科学基金(2008Y02)

摘　　要：用AFLP分子标记技术对N+离子束介导玉米总DNA获得的2个稳定遗传至第6代的水稻早熟变异株系C(08-5-121-6-1)和D(08-5-121-6-2)进行分析。首先从64对引物中筛选出10对多态性较好的引物,然后用这10对引物对早熟变异株系C和D分别扩增。结果显示,变异株系C、D和阴性对照水稻B(豫粳6号)与阳性对照玉米A(郑单14)之间的AFLP扩增图谱相似率分别为15.7%、16.2%和11.6%,说明变异株系与对照豫粳6号的AFLP扩增图谱存在显著差异。变异株系C和D与阴性对照豫粳6号相比,分别扩增出50条和58条差异带,其中新带分别为25条和35条,缺失带分别为19条和15条,变异株系C和D分别扩增到与阳性对照玉米相一致的目的带6条和8条,说明变异株系C和D基因组DNA与玉米基因组DNA相似性高于对照水稻。AFLP marker technique was used to study the two stable heritable early maturing mutant lines C（08-5-121-6-1） and D（08-5-121-6-2） which were developed by N^＋ ion beam mediated maize total DNA into rice Yujing 6.10 pairs of selective primers with high polymorphic bands were selected from 64 pairs of AFLP primers.The results showed that the similarity ratio of amplified bands in mutant lines（C and D） and negative control B（Yujing 6） with the positive control A（maize Zhengdan 14） were 15.7%,16.2% and 11.6%,respectively,which indicated that the amplified bands between mutant lines and control Yujing 6 had significant differences.Compared with negative control,50 and 58 AFLP polymorphic bands were amplified in each mutant line,with 25 and 35 new bands,19 and 15 absent bands in each mutant line respectively.Besides,there were 6 and 8 target bands that also presented in maize were detected in two mutant lines C and D,which revealed that the similarity of genome DNA in mutant lines compared with maize were higher than that of negative control rice.

关 键 词：离子束介导 水稻 AFLP 变异 

分 类 号：S512.1[农业科学—作物学]