沙枣组织培养与快速繁殖  

Tissue culture and rapid propagation of Elaeagnus angustifolia

作　　者：董晓刚[1] 李青梅[1] 陈士刚[1] 李娟[1] 陶晶[1] 

机构地区：[1]吉林省林业科学研究院,吉林长春130033

出　　处：《吉林林业科技》2010年第4期10-13,共4页Journal of Jilin Forestry Science and Technology

摘　　要：以沙枣的顶芽和腋芽为外植体,经过不同时间的灭菌处理,在MS基本培养基中添加不同配比的植物生长调节剂进行离体培养。结果表明:沙枣外植体采用10%Ca(ClO)2上清液处理的最佳灭菌时间为11 min。芽诱导适宜的培养基为MS+6-BA2.0 mg.L-1+NAA2.0 mg.L-1+蔗糖30.0 g.L-1+琼脂4.5 g.L-1,适宜的增殖培养基为MS+6-BA1.5 mg.L-1+NAA1.0 mg.L-1+蔗糖30.0 g.L-1+琼脂4.5 g.L-1和MS+6-BA1.0 mg.L-1+NAA0.5 mg.L-1+蔗糖30.0 g.L-1+琼脂4.5 g.L-1,适宜的生根培养基为1/2MS+NAA0.5 mg.L-1+蔗糖20.0 g.L-1+琼脂4.5 g.L-1。以腐叶土:炉灰=2:1的营养土做移栽基质效果最好,移栽成活率达85%。We used the terminal and axillary buds of Elaeagnus angustifolia as the explants to do in vitro culture after different sterilization times. The MS was used as basic culture medium mixed with different plant growth regulators. The result showed that the best sterilization time of 10% Ca（ClO） 2 liquor was 11min. Culture medium for the induction of bud was MS ＋ 6 -BA2.0 mg· L^-1 ＋ NAA 2.0 mg · L^-1 ＋ saccharose30.0 g · L^-1 ＋ agar 4.5 g · L^-1. Culture medium for the proliferation was MS ＋ 6 -BA 1.5 mg · L^-1 ＋ NAA 1.0 mg · L^-1 saccharose 30. 0 g · L^-1＋ agar 4.5 g · L^-1. Culture medium for the propagation was MS ＋ 6 -BA 1. 0 mg · L^-1 ＋ NAA 0.5 mg · L^-1＋ saccharose 30. 0 g · L^-1 ＋ agar 4.5 g · L^-1. Culture medium of the highest root rate was 1/2 MS ＋ NAA 0.5 g · L^-1 ＋ saccharose 20.0 g · L^-1 ＋ agar 4.5 g · L^-1＋ Making nutrient soil matrix（ leaf mold ： ash = 2 ： 1） to do transplant, we gained survival rate of 85%.

关 键 词：沙枣 组织培养 灭菌 快繁 

分 类 号：S722.37[农业科学—林木遗传育种]