天门冬多糖对小鼠脾脏淋巴细胞体外增殖的影响  

The Effect of Asparagus Cochinchinensis Polysaccharide on Proliferation of Splenic Lymphocytes from Mice in vitro

作　　者：康乐[1] 姚东璧[1] 胡庭俊[1] 李晓栩[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005

出　　处：《中国畜牧兽医》2010年第10期15-18,共4页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30860209);广西"新世纪十百千人才工程"专项资金(2006207)

摘　　要：本试验旨在研究天门冬多糖对小鼠脾脏淋巴细胞体外增殖的影响。从正常小鼠分离脾脏淋巴细胞体外培养,在细胞培养体系中分别加入终浓度为50、100、200、400 mg/L天门冬多糖或配合刀豆素(5 mg/L)作用24、487、2 h,以MTT法测定细胞的增殖程度。结果表明,天门冬多糖可显著促进体外培养的小鼠脾脏淋巴细胞增殖,并与ConA有协同作用。To investigate immunomodulatory effect of Asparagus cochinchinensis polysaccharide（ACP）,the murine splenic lymphocytes cells were incubated in RPMI-1640 tissue culture medium supplemented with ConA（final concentration 5 mg/L）.ACP was added at concentrations of 0,50,100,200 or 400 mg /L on 24,48,72 h,respectively.The cell proliferation were observed.The results showed that ACP was able to stimulate proliferation of splenic lymphocytes within 50 to 400 mg/L.

关 键 词：天门冬 多糖 小鼠 淋巴细胞 增殖 

分 类 号：S816.7[农业科学—饲料科学]