高原型藏绵羊KAP基因单核苷酸多态性分析  

Analysis on Single Nucleotide Polymorphisms of Keratin-associated Proteins Gene in Plateau Tibetan Sheep

作　　者：王志有[1,2] 祁全青[3] 陈玉林[1] 

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100 [2]青海大学农牧学院,西宁810016 [3]青海省三角城种羊场,刚察812300

出　　处：《中国畜牧兽医》2010年第10期120-124,共5页China Animal Husbandry & Veterinary Medicine

基　　金：高产绒量转基因绒山羊新品种培育(2008ZX08008-002);青海大学中青年基金(2009-QN-10)

摘　　要：试验旨在寻找角蛋白辅助蛋白(keratin-associated proteins,KAP)基因与产毛性状相关的多态位点,为藏绵羊产毛性状的分子标记提供理论依据。采用PCR-SSCP技术对高原型藏绵羊角蛋白辅助蛋白基因家族中KAP3.2、KAP7基因部分序列和KAP8基因外显子进行多态性分析。结果表明,3个基因座都存在多态性,均检测到AA、AB和BB 3种基因型。KAP3.2基因以A等位基因为优势等位基因,AA基因型为优势等位基因型;KAP7和KAP8基因均以B等位基因为优势等位基因,BB基因型为优势等位基因型。高原型藏绵羊在KAP3.2和KAP8基因座达中度多态,而在KAP7基因座为低度多态,在KAP3.2和KAP8位点上基因型频率处于Hardy-Weinberg非平衡状态。测序分析结果表明,KAP3.2和KAP8基因上分别存在1个C→T(271 bp)和1个T→C(1122 bp)的突变,均属于同义突变,KAP7基因5′调控区存在1个T→C(102 bp)的突变。This study was conducted to analyze the polymorphisms of keratin-associated proteins（KAP） gene and aid to provide a theoretical foundation for molecular marker of wool production performance in tibetan sheep.The polymorphisms of partial sequence of KAP 3.2,KAP 7 and the exon of KAP 8 in plateau tibetan sheep were analyzed using PCR-SSCP.The results showed that there were polymorphisms in three gene locus,and were detected three genotypes（AA,AB,BB）.The frequency of A allele was obviously higher than that of B allele,and the frequency of AA genotype was higher than AB and BB at KAP 3.2 locus,while the frequency of B allele was higher than A allele,and the frequency of BB genotype was higher than AA and AB at other two gene loci.The polymorphism was medium in KAP 3.2 and KAP 8 gene loci,while low in KAP 7 locus.The frequencies of genotype were deviated from the Hardy-Weinberg disequilibrium at KAP 3.2 and KAP 8 gene loci.The sequencing results showed that there was one mutation at 271 bp of KAP 3.2 and 1122 bp of KAP 8 gene,respectively,and there was one mutation at 102 bp of upstream regulatory region of KAP 7 gene.

关 键 词：高原型藏绵羊 角蛋白辅助蛋白基因 单核苷酸多态性 PCR-SSCP 

分 类 号：S826[农业科学—畜牧学]