利用SSR分析中国北方野生黄花苜蓿种群的遗传多样性  

Genetic Diversity Analysis of Medicago falcata in North China Based on SSR Markers

作　　者：苏东[1] 周延林[1] 于林清[2] 常秀清 

机构地区：[1]内蒙古大学生命科学学院,内蒙古呼和浩特010021 [2]中国农业科学院草原研究所,内蒙古呼和浩特010010 [3]内蒙古四子王旗农业局植保站,内蒙古011800

出　　处：《中国草地学报》2010年第5期85-90,共6页Chinese Journal of Grassland

基　　金：内蒙古草地生态学重点实验室开放基金"黄花苜蓿野生种群遗传多样性地理分布格局及形成机理分析"(2007-01);国家自然科学基金"杂花苜蓿杂种优势及其遗传标记分析"(30371487)

摘　　要：采用SSR分子标记技术对来自内蒙古、新疆两地15个野生黄花苜蓿种群的150个单株的遗传多样性参数进行分析,结果表明：筛选出的12对SSR引物共扩增出135个位点,多态位点131个,多态位点比率为97.04%;15个野生黄花苜蓿种群遗传距离在0.0329～0.2840之间,遗传距离差异较大,说明野生黄花苜蓿种群遗传分化程度较高;UPGMA聚类结果表明,全部内蒙古居群个体与新疆居群个体明显分开,但两大居群中的不同种群个体并未完全聚在一起,呈相互渗透趋势。SSR was applied to analyze genetic diversity of 150 individuals in 15 wild Medicago falcata populations,which were from Xinjiang and Inner Mongolia.135 loci were amplified using 12 pairs of SSR primers,among which 131 were polymorphic locus.The percentage of polymorphic loci was 97.04%.The genetic distances between 15 wild populations were from 0.0329 to 0.2840,showing a high degree of genetic differentiation among population.The UPGMA analysis showed that all the Xinjiang and Inner Mongolia individuals were clearly separated,but the individuals in one population were not clustered entirely in a group,exhibiting a trend of mutual introgression.

关 键 词：SSR 黄花苜蓿 遗传多样性 

分 类 号：S551.7[农业科学—作物学]