猫视网膜多巴胺能神经元的形态和发育  

MORPHOLOGY AND DEVELOPMENT OF DOPAMINERGIC NEURONS IN THE CAT RETINA

作　　者：王厚华[1] 朱慧[1] 吕建利[1] 

机构地区：[1]中国科学院上海生理研究所

出　　处：《实验生物学报》1990年第4期441-451,共11页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金

摘　　要：本文报道用酪氨酸羟化酶(TH)免疫细胞化学方法研究猫生后发育期多巴胺(DA)能神经元的形态和发育。TH阳性反应的DA能神经元在生后第一天(P_1)的视网膜中已经出现。按形态学特征——胞体大小、形状、突起分层,以及免疫反应强度可分为THⅠ型和THⅡ型两类。THⅠ型是大的强阳性反应的DA能细胞,包括通常DA能无长突细胞、移位DA能无长突细胞和DA能类网间细胞。它们随发育年龄增长逐渐成熟。THⅡ型是小的弱阳性反应的DA能细胞,不随年龄而生长发育,相反在开眼(P_(7-10))后细胞数量明显下降,至P_(30)时完全消失。开眼后,THⅠ细胞除胞体增大外树突发育特别迅速。它们的胞体直径从11.8μm(P_1时)增大至14.2μm(P_(30)时),相应的树突野和分枝交叉也明显增加。P_1时,树突分枝少而直,末端有许多生长锥。在中央网膜的树突有棘状附属物。至P_(13)时生长锥减少,许多分枝交叉形成简单的网状,同时树突“棘”完全消失,可能发展为环的一部份。至P_(30)时,树突分枝在内网从层的外层形成复杂的网络,其间有无数与杆型AⅡ无长突细胞构成突触联系的环形结构,相似于成年者。在生后发育过程中,开眼后适宜光照对THⅠ细胞成熟的影响以及神经递质变化的可能性,我们在文中进行了讨论。Morphology and development of dopaminergic neurons has been studied in the kitten retina, using tyrosine hydroxylase (TH) immunocytochemistry. TH immunoreactive (TH^+) cells are already presented in wholemount and sectioned retina at first postnatal day (P 1). Aceqrding to soma size, shape, dendritic process pattern and immunoreactivity, two classes, type I or large dark staining TH^+ cells and type Ⅱ or small light staining TH^+ cells are recorgnized. The TH Ⅰ cells which consisting of normal placed DA amacrine ceils, displaced DA amacrine cells and DA interplexform-like cells, graduately mature during postnatal development, while TH Ⅱ cells decrease quickly and throught disappear at P30.After eye opening TH Ⅰ amacrine cells, especially their dendrites develop quickly. The soma diameters increase from 11.8μm (P1) to 14.2μm (P30). The dendritic fields increase in size and complexity. At P1 the thick radiating dendrites emerge from the cell body with small or large 'spines' and many growth cones. At P 13 the dendritic field is markedly enlarged and only a few growth cones can be seen on some stained dendrites. In addition, the dendritic spines are no longer appearent and they are a part of rudimentary rings. By P 30 the dendritic plexus of TH^+ dendrites and rings in the outmost part of IPL, typical of the adult cells, are complete. The influence of light on the development of DA cells after eye opening and the possibility of neurotransmetter changing are discussed.

关 键 词：猫 视网膜 DA能神经元 生后发育 

分 类 号：Q959.838[生物学—动物学]