巴马香猪TLR2基因cDNA的克隆及生物信息学分析  

Cloning and bioinformatics analysis of TLR2 cDNA in Bama miniature pig

作　　者：徐汉进[1] 雍艳红[2] 安立龙[1] 巨向红[2] 

机构地区：[1]广东海洋大学动物科学系,广东湛江524088 [2]广东海洋大学动物医学系,广东湛江524088

出　　处：《中国兽医学报》2010年第7期949-953,共5页Chinese Journal of Veterinary Science

基　　金：广东海洋大学博士启动基金资助项目(0712107)

摘　　要：利用NCBI公布的TLR2基因序列设计引物,RT-PCR克隆巴马香猪(Sus barbatus)TLR2基因。核酸序列分析表明,巴马香猪TLR2基因开放阅读框长2358bp,编码785个氨基酸,该蛋白等电点为7.52,相对分子质量为89480;与普通猪比对发现,巴马香猪TLR2基因有15个碱基发生突变;与小鼠、狗、鸡、牛、羊和人的同源性分别为73.4%,82.4%,59.5%,85.3%,84.6%,82.4%。TLR2膜外区蛋白为背侧多个α螺旋和内侧多个β折叠平行交替排列构成一个弯曲状螺旋结构;N末端存在信号肽,且可能在21～22位氨基酸处存在裂解位点;胞外区有4个明显的LRR,分别位于第76～99、360～385、413～432和457～476位氨基酸区;膜外区含6个N连接的糖基化位点。Toll-like receptor 2 （TLR2） from peripheral blood mononuclear cell in Bama miniature pigs was cloned by RT-PCR and some characteristic of TLR2 protein was predicted in the present study.The results showed that receptor TLR2 gene consists of 2 358 base pairs,coding 785 amino acids.The molecular weight and the isoelectric point of TLR2 protein was 89 480 and 7.52,respectively,and having a transmembrane region（589-611） and a signal peptide.15 bases of TLR2 gene in Bama miniature pig mutated compared with general pig,but the structure does not change significantly.Bama miniature pig TLR2 gene sequence has high homology compared with mice（73.4%）,dog（82.4%）,chicken（59.5%）,cattle（85.3%）,sheep（84.6%） and man（82.4%）.The structure of extracellular region of TLR2 protein was forniciform arc,and consisted of a lot of α-helix in inside and β-sheet in outside of arc.There are four leucine-rich repeats and six N-linked glycosylation sites out of the membrane.

关 键 词：巴马香猪 TLR2基因 生物信息学分析 

分 类 号：S852.4[农业科学—基础兽医学] Q78[农业科学—兽医学]