活体电穿孔方法提高外源GRF基因在小鼠肌肉组织中的表达  

In vivo electroporation enhances foreign GRF gene expression efficiency in skeletal muscle of mice

作　　者：齐剑英[1] 刘松财[1] 戴建威[1] 丁景华[2] 杨丽华[2] 江青艳[2] 张永亮[1,2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]华南农业大学动物科学学院,广东广州510624

出　　处：《中国兽医学报》2010年第7期954-957,961,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30270973)

摘　　要：向小鼠后肢小腿肌注pGRF表达质粒并加以电穿孔条件,注射剂量分别为5,10,50μg,于注射前及注射后10,20,30d测体质量,并采血分离血清测血清GRF水平。结果显示,5μgpGRF质粒电穿孔组注射后10dGRF分别比对照组、5μgpGRF质粒组升高44.73%(P<0.05),12.86%(P>0.05);血清中GRF水平升高。30d累积增重,5μgpGRF质粒电穿孔组分别比对照组、5μgpGRF质粒组高11.46%,6.75%(P>0.05);10μgpGRF质粒组分别比对照组和10μgpGRF质粒电穿孔组高19.52%(P>0.05),19.42%(P>0.05)。50μgpGRF质粒组45d累积增重分别比对照组、pGRF质粒电穿孔组高14.00%,12.00%(P>0.05)。结果表明,电穿孔处理可提高GRF基因在小鼠肌肉中的表达。The GRF gene plasmids（pIRES-GRF and pcDNA3-GRF） were injected into skeletal muscle of mouse by electroporation in vivo.The dosage of injection was 5,10,50 μg GRF gene plasmid,respectively.The result showed that at 10 d postinjection,concentrations of serum GRF of mice in the group injected with 5 μg pGRF by electroporation in vivo was 44.73%（P0.05） higher than the control,and 12.86%（P0.05） higher than the group injected with 50 μg pGRF.At 30 d postinjection,accumulative increased body weights in the group injected with 5 μg pGRF by electroporation in vivo was 11.46%（P0.05） higher than the control and 6.75%（P0.05） higher than the group injected with 5 μg pGRF.The accumulative increased body weights in the group injected with 10 μg pGRF directly was 19.52%（P0.05） higher than the control,and 19.42%（P0.05） higher than the group injected with 10 μg pGRF by electroporation in vivo.After 45 d,accumulative increased body weights in the group injected with 50 μg pGRF directly was 14.00%（P0.05） higher than the control,and 12%（P0.05） higher than the group injected with 50 μg pGRF by electroporation in vivo.The results suggested that in vivo electroporation was able to enhance GRF expression in mouse skeletal muscle in a strict manner.

关 键 词：活体电穿孔 生长激素释放因子 小鼠 肌肉 

分 类 号：Q78[生物学—分子生物学]