β-谷甾醇、淫羊藿苷对猪精子体外获能效果的影响  

Effect of β-sitosterol and icariin on porcine spermatozoa capacitation in vitro

作　　者：赵曌[1] 徐捷[1] 相荣科[1] 赵菊[1] 刘笑然[1] 吴波[1] 王占赫[1] 高建明[1] 

机构地区：[1]北京农学院动物科学技术系,北京102206

出　　处：《中国兽医学报》2010年第7期996-999,共4页Chinese Journal of Veterinary Science

基　　金：北京市教委资助项目(KM200710020014)

摘　　要：探讨中药单体成分β-谷甾醇(β-sitosterol)、淫羊藿苷(Icariin,Ica)对猪精子体外获能的影响,以期提高体外受精效果。以mTBM+肝素获能液为对照组,分别添加β-谷甾醇、淫羊藿苷的低、中、高3个剂量为试验组,利用金霉素荧光染色(Chlortetracycline,CTC)法结合体外受精检验体外获能效果。在mTBM+肝素中添加β-谷甾醇2(中剂量0.08mg/L)、淫羊藿苷2(中剂量17mg/L)的B型精子率(获能精子)显著高于对照组和其他剂量组(P<0.05),F型精子率(未获能精子)显著低于对照组和其他剂量组(P<0.05),AR型精子率(发生顶体反应精子)与对照组和其他剂量组差异不显著(P>0.05),卵裂率和囊胚率均极显著高于对照组和其他剂量组(P<0.01);表明中药单体成分β-谷甾醇、淫羊藿苷能够显著促进猪精子体外获能效果。To investigate the effect of adding two kinds of chinese tradional madicine monomer β-sitosterol and icariin on porcine spermatozoa capacitation in vitro.The discrepancy effect on porcine spermatozoa capacitation in vitro between β-sitosterol and icariin was compared by using chlortetracycline （CTC） staining and in vitro fertilization （IVF）.The results showed that β-sitosterol 2（0.08 mg/L） and icariin 2（17 mg/L） groups could improve the effect of capacitation in vitro.In experiment 1,the rate of porcine spermatozoa capacitation in vitro （38.53%） in β-sitosterol 2 group was higher than the other groups.The cleavage rate （76.32%）,blastocyst rate （24.50%） of β-sitosterol 2 group were extremely higher than the other groups.There was no statistical difference between the rest （P0.05）.In experiment 2,the rate of porcine spermatozoa capacitation in vitro （40.89%） in Ica 2 group was higher than the other groups.The cleavage rate （78.58%）,blastocyst rate （26.67%） of Ica 2 group were extremely higher than the other groups.There was no statistical difference between the rest of groups（P0.05）.In conclusion,the results showed that adding two kinds of Chinese tradional madicine monomer β-sitosterol and icariin could obviously improve the effect of porcine spermatozoa capacitation in vitro.

关 键 词：Β-谷甾醇 淫羊藿苷 体外获能 猪精子 

分 类 号：S814.8[农业科学—畜牧学]