Cr^(6+)胁迫对大豆幼苗生理生化特性的影响  

Effects of Cr^(6+) Stress on Physiological and Biochemical Parameters of Soybean Seedlings

作　　者：李三相[1] 李勃[1] 兰小平[1] 何增林[1] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741001

出　　处：《大豆科学》2010年第5期777-780,共4页Soybean Science

基　　金：天水师范学院青年基金资助项目;甘肃省自然科学基金资助项目(3ZS051-A25-026)

摘　　要：在培养箱中用含有不同浓度K2Cr2O7的Hoagland营养液处理有4片叶子的大豆幼苗,8 d后测量其生理指标以揭示Cr6+对大豆幼苗毒害机制。结果表明:低浓度K2Cr2O7(5 mg.L-1)对大豆幼苗的生长有促进作用,与对照相比,其株高、叶绿素含量分别增加了4.97%、18.2%,MDA含量降低了15.95%,POD活性差异不显著;而高浓度K2Cr2O7(10～100 mg.L-1)对大豆幼苗有显著毒害作用,随K2Cr2O7浓度的增加,其株高、叶绿素含量显著降低,脯氨酸含量升高、细胞膜透性增大,POD活性显著降低,幼苗生长受到抑制。In order to study toxic mechanism of Cr6＋ to plant, soybean seedlings with four leaves were cultured in Hoagland＇s nutrient solution that containing different concentration of Cr6 ＋ . Physiological functions of soybean seedlings were investigated after eight days treatment. The results showed that Cr6＋ with low concentration （5 mg ·L -1 ） stimulated the growth of soybean seedlings. Height and total chlorophyll content of soybean seedlings increased by 4.97% and 18.2% respectively after 5 mg · L-1 Cr6＋ treatment. Moreover, MDA content decreased 15.95% and the POD activity did not have significant difference compared with control, however, Cr6＋ with high concentration（ 10- 100 mg · L-1）inhibited the growth of soybean seed- lings, plant height, total chlorophyll content and POD activity of soybean seedlings all decreased, however, proline content and permeability of cell membranes increased after high concentration Cr6 ＋ treatments.

关 键 词：Cr6+ 大豆幼苗 生理特性 生长 

分 类 号：S565.1[农业科学—作物学]