大豆疫霉根腐病菌毒素处理不同大豆品种后过氧化物酶活性的变化  

Changes of Peroxidase in Soybean Treated by Pathotoxin Produced by Phytophthora sojae

作　　者：陈晨[1] 徐鹏飞[1] 张淑珍[1] 王萍[2] 吴俊江[3] 李宁辉[1] 范素杰[1] 王欣[1] 李文滨[1] 

机构地区：[1]东北农业大学大豆研究所大豆生物学教育部重点实验室,黑龙江哈尔滨150030 [2]淮海工学院海洋学院,江苏连云港222005 [3]黑龙江省农业科学院大豆研究所,黑龙江哈尔滨150086

出　　处：《大豆科学》2010年第5期840-844,共5页Soybean Science

基　　金：国家自然科学基金资助项目(30400285;30671317;30810103063;30972889);黑龙江省自然科学基金资助项目(C200814);中国博士后基金资助项目(20060400835);黑龙江省新世纪优秀人才培养计划资助项目(NCET-06-007);黑龙江省高校骨干教师资助项目(1151G007);黑龙江省农业科学院博士后基金资助项目(LRB06-010);博士后落户黑龙江科研启动基金资助项目(LBH-Q05032);农业部寒地重点开放实验室开放课题

摘　　要：大豆疫霉根腐病是危害大豆生产的世界范围的毁灭性病害,毒素在其致病过程中起着重要作用。对大豆疫霉根腐病菌毒素胁迫下抗感不同大豆品种根、茎、叶中过氧化物酶(POD)活性的变化进行了初步研究。结果表明:适宜浓度的毒素(稀释100倍,浓度为0.0897 mg.mL-1)处理后,抗病大豆品种根、茎和叶中POD活性在病程的大部分阶段与对照相比都有升高,并且根中比茎叶中的POD反应更敏感;感病品种虽然在某些病程阶段POD活性较对照有一定的提高,但幅度不大,在病程其它阶段POD下降幅度远大于升高幅度。而浓度相对较高的毒素(稀释50倍,浓度为0.1794 mg.mL-1)处理后抗感品种根、茎和叶中POD活性的变化较稀释100倍浓度毒素幅度小。Phytophthora root and stem rot caused by Phytophthora sojae is a worldwidly destructive soybean disease, and the pathotoxin produced by P. sojae is an important pathogenic factor in the pathogenicity process. In this study, changes of per- oxidase （POD） of nine soybean varieties with different resistance treated with the pathotoxin was preliminarily researched, and the results were as follows： The POD activities in roots, stems, and leaves of resistant soybean varieties treated with the pathotoxin of optimal concentration （ Diluted to 100 times, and the concentration is 0. 0897 mg · mL- 1 ） was higher than that of control at most of the pathogenicity stages, and the reaction of POD in roots is more sensitive than that in stems and leav- es. The POD activities in susceptible soybean varieties increased compared with that in control at some of the pathogenicity stages, however, it decreased at most of the pathogenic stages. Treated with pathotoxin with high concentration （Diluted to 50 times, and the concentration is 0. 1794 mg ·mL-1 ） , the change extent of POD activity in roots, stems, and leaves in the nine soybean varieties was relatively low. The results indicated that there was difference in POD activities among different soybean cultivars treated with optimal concentration pathotoxin produced by P. sojae.

关 键 词：大豆 大豆疫霉根腐病 毒素 过氧化物酶 

分 类 号：S565.1[农业科学—作物学]