HPLC法测定肿节风药材中异秦皮啶和迷迭香酸的含量  

Determination of Isofraxidin and Rosmarinic Acid in Sarcandra glabra by HPLC

作　　者：李婷婷[1,2] 肖小武[1,2] 宁火华 罗跃华 汪玢[1] 

机构地区：[1]南昌大学药学系,江西南昌330046 [2]江西省食品药品检验所,江西南昌330029

出　　处：《南昌大学学报（理科版）》2010年第4期358-360,共3页Journal of Nanchang University(Natural Science)

基　　金：江西省科技厅基金资助项目(2007BS12801);江西省卫生厅基金资助项目(20082020)

摘　　要：建立了肿节风中异秦皮啶和迷迭香酸含量的测定方法。以乙腈-0.1%磷酸溶液（20：80,V/V）为流动相,流速为1.0 mL/min,柱温35℃,UV检测波长为342 nm。样品经0.45μm微孔滤膜过滤后,按选定的色谱条件进行分析,异秦皮啶和迷迭香酸分别在0.011~2.214μg/mL、0.022~2.198μg/mL范围内线性关系良好,相关系数均为0.999 0。方法的加标回收率为102.57%~102.94%。该方法灵敏快速,操作简便,重现性好,可用于肿节风药材的质量控制。The high-performance liquid chromatography（HPLC） detection method was developed for the determination of isofraxidin and rosmarinic acid in Sarcandra glabra.The conditions of HPLC were the following： the mobile phase was acetonitrile-0.1% phosphoric acid solution（80：20,V/V）,the flow rate was 1.0 mL/min,the column temperature was 35 ℃,the detection wavelength was 342 nm.The liner range was 0.011-2.214 μg/mLand 0.022-2.198 μg/mL of isofraxidin and rosmarinic acid,with the correlation coefficients of 0.999.The average recoveries of the two ingredient were 102.57% and 102.94% correspondingly in Sarcandra glabra.The method was successfully applied to the detemination of isofraxidin and rosmarinic acid in Sarcandra glabra.

关 键 词：肿节风 异秦皮啶 迷迭香酸 HPLC 

分 类 号：R917[医药卫生—药物分析学]