提莫菲维小麦与二倍体野燕麦远缘杂交后代的DNA斑点杂交分析  

DNA Dot Hybridization Analysis of Distant Filial Generation from T. timopheevi×Diploid Wild Oat

作　　者：刘利青[1] 周朝廷[1] 周海鹏[1] 张素勤[1] 耿广东[1] 张庆勤[1] 

机构地区：[1]贵州大学农学院,贵州贵阳550025

出　　处：《贵州农业科学》2010年第10期1-3,共3页Guizhou Agricultural Sciences

基　　金：贵州省农业攻关项目"小麦种质资源创新与利用"[黔科合NY字(2008)3017]和"小麦远缘杂交后代种质的评价与利用"[黔科合带帽字(2009)5002];贵州省优秀科技教育人才省长基金"提莫菲维小麦与野燕麦遗传背景研究及其远缘杂交后代遗传分析"[黔省专合字(2007)19]

摘　　要：为了鉴定提莫菲维小麦与二倍体野燕麦属间远缘杂交后代的真实性,以地高辛标记的二倍体野燕麦DNA为探针、提莫菲维小麦为封阻剂对其远缘杂交后代进行了DNA斑点杂交分析。结果表明:杂交液中使用的探针浓度为25 ng/mL,探针与封阻DNA比例为1∶100时可完全避免假阳性现象,杂交后代出现了蓝紫色斑点杂交信号。提莫菲维小麦与二倍体野燕麦杂交后代中含有二倍体野燕麦的遗传物质,在一定程度上证实了其远缘杂交后代的真实性。The DNA dot hybridization of distant filial generations from T.timopheevi×diploid wild oat was analyzed by using DNA of diploid wild oat with a digoxigenin marker as probe and using T.timopheevi as a betablocker to identify the truth of genus cross between T.timopheevi and diploid wild oat.The results showed that the false positive phenomenon could be avoided completely and the bluish violet dot hybridization signal appeared when the probe concentration was 25ng/mL and the probe-betablocker ratio was 1∶100,which indicates that there is genetic material of diploid wild oat in the filial generation from T.timopheevi× diploid wild oat and confirms the truth of its distant hybridization to a certain extent.

关 键 词：提莫菲维小麦 二倍体野燕麦 远缘杂交 斑点杂交 

分 类 号：S512.1[农业科学—作物学]