不同消毒方式及生长调节剂浓度对红双喜月季组培的影响  

Effect of Different Sterilization Pattern and Growth Regulator Concentration on Tissue Culture of Chinese Rose

作　　者：刘会超[1] 郭丽娟[1] 贾文庆[1] 刘孟刚[1] 

机构地区：[1]河南科技学院,河南新乡453003

出　　处：《贵州农业科学》2010年第10期14-17,共4页Guizhou Agricultural Sciences

基　　金：河南省创新人才工程项目(2005-126-49);河南科技学院博士基金(050106)

摘　　要：为了对红双喜月季的组织快繁培养和工厂化育苗提供参考,以红双喜茎段为外植体,研究了外植体消毒方式、培养基类型以及生长调节剂NAA、6-BA等因素对腋芽诱导、腋芽生长、增殖及生根的影响。结果表明,消毒效果以75%酒精处理外植体1min,再用0.1%HgCl2消毒8min效果最好;腋芽萌发率以20mg/L6-BA和0.01mg/L NAA混合作用最高,达76.19%;6-BA和NAA能有效提高芽的增殖系数,以1.5mg/L6-BA和0.1mg/L NAA混合作用芽的增殖系数最高,达2.24;低浓度的无机盐(1/2 MS)和低浓度的生长调节剂(0.01mg/L IBA)混合作用有利于红双喜根的分化和生长。The explants of stem section of Hongshuanxi（a Chinese rose variety） were cultured on different media with different hormone combination and concentration by using different sterilization patterns to study the effect of sterilization pattern,hormone combination and concentration on induction,growth,propagation and rooting of axillary buds of cultured explants.The optimum sterilization pattern is that explants are firstly dipped in 75% alcohol for 1 min and then sterilized by 0.1 %HgCl2 for 8 min.The germination rate of axillary buds of the explants cultured on MS ＋ 20 mg/L 6-BA ＋ 0.01 mg/L NAA is the highest（76.19%）.The propagation coefficient of axillary buds cultured on MS ＋ 1.5 mg/L6-BA ＋ 0.1 mg/LNAA is the highest（2.24）.1/2 MS ＋ 0.01mg/L IBA is beneficial to root differentiation and growth of plantlets.

关 键 词：月季 红双喜 茎段 组织培养 快繁 

分 类 号：S685.12[农业科学—观赏园艺]