序贯法测定大鼠腹腔注射苯巴比妥钠和丙泊酚的麻醉半数有效量  

The median effective dose of propofol and phenobarbital sodium for general induction was measured by up-down method

作　　者：李振洲[1] 陈学新[2] 孟尽海[2] 邓丽琴[2] 闫瑞[1] 王培栋[1] 

机构地区：[1]宁夏医科大学,宁夏银川750004 [2]宁夏医科大学附属医院,宁夏银川750004

出　　处：《宁夏医学杂志》2010年第10期867-869,共3页Ningxia Medical Journal

基　　金：宁夏自然科学基金资助项目(NZ10114)

摘　　要：目的序贯法测定SD大鼠腹腔注射苯巴比妥钠和丙泊酚的麻醉半数有效量(ED50),并确定两种药物的效价比。方法 36只SD大鼠,随机分为两组:苯巴比妥钠组(n=18),丙泊酚组(n=18)。大鼠腹腔给药后立即观察大鼠的行为学表现,以大鼠前爪翻正发射消失作为麻醉终点的指标,前爪翻正反射恢复作为意识恢复的指标。用序贯增减法确定给药剂量,根据公式ED50=lg-1(ΣC/Σt)计算各组动物的ED50,在此基础上求出两药ED50的效价比。结果序贯法测定SD大鼠苯巴比妥钠的麻醉半数有效量ED50为122.415mg/kg;丙泊酚的麻醉半数有效量ED50为59.452mg/kg,两种药物ED50的效价比为0.486。结论序贯法测定药物的半数有效量简便、高效、可信。应用序贯法测定了正常大鼠通过腹腔注射苯巴比妥钠和丙泊酚的ED50,并得出苯巴比妥钠和丙泊酚的效价比是0.486。Objective To measured median effective dose（ED50） of phenobarbital sodium and the propofol by up-down method via administered intraperitoneally to SD rats.Methods 36 rats were divided into two group randomly： phenobarbital group（group PB,n=18） and propofol group（group Pr,n=18）.Drugs were intraperitoneally injected to SD rats（BW 180-220g） and observed the behaviors of the rats.The loss of forepaw righting reflex（FRR） was considered as successful anesthesia induction,the recovery of forepaw righting reflex（FRR） was considered as regain consciousness.Determined the dosage with sequential change,the ED50 with formula of ED50=lg-1（ΣC/Σt） and the potency ratio on them was calculated.Results The ED50 of phenobarbital and propofol were respectively 122.415mg/kg and 59.452mg/kg.The potency ratio for phenobarbital and propofol was 0.486.Conclusion This up-down method is simple,highly selective and efficient for measuring the ED50.Phenobarbital sodium and the propofol ED50 was measured with up-down method by intraperitoneally injection.The potency ratio for pentobarbital and propofol was 0.486.

关 键 词：序贯法 苯巴比妥钠 丙泊酚 ED50 

分 类 号：R614.2[医药卫生—麻醉学]