甲状旁腺素相关肽对INS-1细胞株胰岛素分泌的影响  

PTHrP induced insulin secretion and production in INS-1

作　　者：梁华晟[1] 薛耀明[2] 钟宇华[1] 

机构地区：[1]广西医科大学第九附属医院内分泌科,广西北海536000 [2]南方医科大学南方医院内分泌科,广东广州510515

出　　处：《重庆医学》2010年第20期2732-2733,2736,共3页Chongqing medicine

基　　金：广西科技厅自然科学基金资助项目(桂科自NO.0991294);广西北海市科学研究与技术开发计划资助项目(北科合字200901059)

摘　　要：目的探讨甲状旁腺素相关肽(PTHrP)对大鼠胰岛β细胞瘤株INS-1胰岛素含量的影响。方法根据不同干预条件分为空白对照组、PTHrP干预组(分别应用1、10、100 pmol/L的PTHrP干预),48 h后应用放射免疫法检测各组葡萄糖刺激胰岛素分泌作用,Fura-3/AM荧光负荷技术测定Ca2+。Western Blot检测细胞胰岛素及胰腺-十二指肠同源框1(PDX-1)的表达。结果 PTHrP对INS-1细胞葡萄糖刺激胰岛素分泌能力及Ca2+负荷呈浓度依赖性关系。PTHrP组上调胰岛素及PDX-1表达,与空白对照组比较差异有统计学意义(P<0.01)。结论 PTHrP可以促进胰岛β细胞胰岛素分泌及合成。Objective To study whether the PTHrP had increased insulin secretion and production in INS-1.Methods Based on different condition,there are two groups had been divided：control group,PTHrP group（1,10,100 pmol/L PTHrP）,which glucose-stimulated insulin secretion（GSIS） was detected by Radioimmunoassay,the expression of insulin and PDX-1 were detected by Western Blot after 48 h.Fura-3/AM was used to measure the fluorescent intensity of Ca2＋.Results The GSIS and fluorescent intensity of Ca2＋ in response to PTHrP in a dose dependent manner,the expression of insulin and PDX-1 in PTHrP group was higher than control group.Conclusion PTHrP can increase insulin secretion and prodution in INS-1.

关 键 词：甲状旁腺素相关肽 胰岛素 INS-1 

分 类 号：R587.1[医药卫生—内分泌]