预先电针三阴交 血海 合谷对痛经大鼠模型子宫IP_3的影响  

Effect on IP_3 in the Uterus Tissue of Dysmenorrhea Model Rats with Pre-Electroacupuncture the "Sanyinjiao" (SP6) "Xuehai" (SP10) "Hegu "(LI4)

作　　者：郭孟玮[1] 丁喜艳[1] 任晓暄[1] 赵雅芳[1] 李春华[1] 嵇波[1] 朱江[1] 张露芬[1] 

机构地区：[1]北京中医药大学针灸学院,北京100029

出　　处：《辽宁中医杂志》2010年第10期2048-2051,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划("973"计划)资助项目(2006CB504503)

摘　　要：目的:观察预先电针同经不同类及不同经脉腧穴对痛经大鼠模型子宫IP3的影响。方法:50只雌性SD大鼠,随机分为盐水组、模型组、电针三阴交组、血海组、合谷组,每组10只。除盐水组外,其余各组大鼠均连续10天给予大鼠皮下注射苯甲酸雌二醇注射液,末次给药后1h,腹腔注射缩宫素2U/只,制造痛经大鼠模型,盐水组每日给予同等剂量的生理盐水。于第8日针刺各组给予电针20min,盐水组及模型组束缚20min,每日1次,共3次,于末次针刺后观察扭体反应,并采用酶联免疫法检测子宫IP3。结果:①每分钟扭体分数:与盐水组比较,模型组、血海组及合谷组均明显升高(P均<0.01);三阴交组无显著性差异(P>0.05)。与模型组比较,三阴交组显著性降低(P<0.05);血海组、合谷组均无显著性差异(P均>0.05)。各电针组之间比较,血海组较三阴交组明显升高(P<0.01)。②子宫IP3水平:与盐水组比较,模型组、血海组、合谷组均显著降低(P<0.01,P<0.05);三阴交无显著性差异(P>0.05)。与模型组比较,三阴交组明显升高(P<0.01);血海组、合谷组均无统计学差异(P均>0.05)。各电针组之间比较,三阴交组明显高于血海组、合谷组(P均<0.01)。结论:三阴交组明显抑制了大鼠扭体反应,其机制是通过对IP3信号转导途径的生理性调节,而产生抑制子宫平滑肌收缩的作用。血海组及合谷组对扭体反应及IP3均未产生明显调节作用,表明同经不同类及不同经脉穴位之间的效应存在差异。Objective：To research the effect on IP3 in the uterus tissue of dysmenorrhea model rats with Pre-electroacupuncture the different classes acupoints in the same meridian and the different meridians apoints.Methods：50 femal SD rat were randomized into saline,model,electroacupuncture（EA）＂Sanyinjiao＂（SP6）,EA＂Xuehai＂（SP10）,EA＂Hegu ＂（LI4）groups with 10 rats in each group.In addition to saline group,the remaining rats groups were given subcutaneous injection of estradiol benzoate injection for 10 consecutive days.1h After the last administration,intraperitoneal injection of oxytocin 2u each rat to create dysmenorrhea rats model,saline group given the same dose of saline per day.On the 8th day each group of acupuncture given EA 20min,saline group and model group were bound 20min,Once a day,a total of 3 times.observing the writhing response after the last acupuncture.The uterine IP3 were detected with enzyme-linked immunosorbent assay method.Results：①Writhing scores per minute：Compared with the saline group,Writhing scores per minute increased significantly in the model,SP10 and LI4 groups（all P〈0.01）;There was no significant difference in SP6 group（P〈0.05）.Compared with the model group,Writhing scores per minute decreased significantly in the SP6 group（P〈0.05）.There were no significant difference in SP10 and LI4 groups（all P〈0.05）.Writhing scores per minute in SP10 group was significantly higher than that in SP6（P〈0.01）②IP3 level of the uterus：Compared with the saline group,model group,SP10 and LI4 groups were lower significantly（P〈0.01,P〈0.05）,SP6 group no significant difference（P〈0.05）.Compared with model group,SP6 was increased significantly（P〈0.01;there was no significant difference in SP10 and LI4 groups（all P〈0.05）;The IP3 level in SP6 was significantly higher than that in SP10 and LI4 groups（all P〈0.01）.Conclusion：Pre-EA SP6 group inhibited the rats writhing response significantly,The mechanisms is to regulat th

关 键 词：痛经 IP3 经穴效应特异性 预先电针 

分 类 号：R246.3[医药卫生—针灸推拿学]