中红外光谱法对肉苁蓉的定性和半定量分析(英文)  

QUALITATIVE AND SEMIQUANTITATIVE ANALYSIS OF Cistanche deserticola BY MID-INFRARED SPECTROSCOPY

作　　者：徐荣[1] 孙素琴[2] 辛士光[3] 朱维成[3] 石钺[1] 刘同宁 陈君[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100094 [2]清华大学化学系,北京100084 [3]哈尔滨理工大学计算中心,哈尔滨150080 [4]宁夏永宁县本草苁蓉种植基地,银川750100

出　　处：《红外与毫米波学报》2010年第5期325-328,350,共5页Journal of Infrared and Millimeter Waves

基　　金：National Natural Science Foundation of China(30772727);National Technology Support Project(2006BAI09B01);national S&T special projects(2009ZX09308-002)

摘　　要：本文尝试将中红外光谱（MIR）应用于肉苁蓉药材的质量评价和分析中.首先比较肉苁蓉药材粉末样品及其3种主要活性成分提取物,包括本乙醇总苷（PeGs）、甘露醇（Man）和多糖的红外光谱图差异,得出3种提取物的特征红外吸收峰,确定药材样品各特征峰的强弱与PeGs和Man含量高低的关系.然后用Spectrum v5.02软件计算得出不同波段内药材图谱与两种提取物图谱的红外相似系数,通过SPSS11.0软件分析发现,药材PeGs的含量（y）和1800~1200cm-1范围内的红外相似系数（x）呈极显著相关（R2=0.9927）,回归方程：y=19.846x＋0.0623.同样,药材Man的含量（y）与1100~600cm-1波数范围的红外相似系数（x）呈极显著相关（R2=0.9705）,回归方程：y=13.855x-0.1714.因此,M IR可作为肉苁蓉药材质量定性和半定量分析的快速有效方法,直观评价药材质量.In the present study,mid-infrared spectroscopy（MIR） was attempted to apply for quality evaluation of C.deserticola.First of all,IR spectra of the herbal sample and its 3 main extracts including PeGs,Man and Polysaccharides were compared.Characteristic absorption peaks within different wave number range were found in different extracts.Then the authors managed to find the relationships between the intensity of the characteristic peaks and the relative content of the PeGs and Man in herbal sample.The IR spectra of the herbal samples were compared with PeGs and Man using Spectrum v5.02 software（Perkin Elmer） for IR Corrdations.It was found that the content of PeGs（y） and its IR Correlation（x） within herbal sample in the range of 1800~1200cm-1 were correlative（R2= 0.9927） by the SPSS 11.0 analysis,and the regression equation is y=19.846x ＋ 0.0623.In the same way,the content of Man（y） is correlative with the IR Correlation（x） in the range of 1100~ 600cm-1（R2 =0.9705）,and the regression equation is y=13.855x-0.1714.Therefore,MIR spectroscopy is a fast and effective method in qualitative and semiquantitative analysis of quality evaluation of C.deserticola.

关 键 词：中红外光谱 肉苁蓉 定性 半定量 质量评价 

分 类 号：R283[医药卫生—中药学]