Fas/FasL系统激活对LPS诱导肺泡Ⅱ型上皮细胞分泌MCP-1、TNF-α的影响  

Effect of Fas/FasL on MCP-1 and TNF-α of Alveolar Type Ⅱ Epithelial Cells Induced by LPS in Rats in Vitro

作　　者：唐芳[1] 徐道妙[1] 马新华[1] 艾宇航[1] 

机构地区：[1]中南大学湘雅医院ICU,湖南长沙410008

出　　处：《实用预防医学》2010年第10期1935-1937,共3页Practical Preventive Medicine

摘　　要：目的探讨Fas/FasL系统对LPS诱导肺泡Ⅱ型上皮细胞分泌炎症因子(MCP-1、TNF-α)的影响。方法原代培养肺泡Ⅱ型上皮细胞,随机分为四组(每组平行6孔):对照组、LPS组、LPS+FasL组、FasL组,加入LPS、FasL调节终浓度为LPS:10μg/ml,FasL:5 ng/ml。培养48 h后,ELISA检测MCP-1、TNF-α浓度。结果与对照组比较,LPS组、FasL+LPS组、FasL组TNF-α与MCP-1浓度增加(P<0.01);与LPS组比较,FasL+LPS组TNF-α与MCP-1浓度增加(P<0.05)。结论 Fas/FasL使LPS诱导的肺泡Ⅱ型上皮细胞MCP-1与TNF-α分泌增加。Objective To study the effect of Fas/FasL on secretion of MCP-1 and TNF-α from rat alveolar type Ⅱ epithelial cells induced by lipopolysaccharide（LPS）.Methods Rat alveolar type Ⅱ epithelial cells in vitro which were isolated,purified and incubated were randomly divided into four groups： control group,LPS group,LPS＋FasL group,and FasL group.LPS and FasL were put into DMEM.The final concentrations of LPS and FasL were 10 ug/ml and 5 ng/ml,respectively.After incubation for 48 hours,the concentrations of MCP-1 and TNF-α were measured by ELISA.Results As compared with control group,the concentrations of MCP-1 and TNF-α of rat alveolar type Ⅱ epithelial cells in vitro were significantly increased in LPS group,FasL＋LPS group and FasL group（P〈0.01）.As compared with LPS group,the concentrations of MCP-1 and TNF-α were significantly increased in FasL＋LPS group（P〈0.05）.Conclusions Fas/FasL can increase secretion of MCP-1 and TNF-α from rat alveolar type Ⅱ epithelial cells induced by LPS.

关 键 词：肺泡 上皮细胞 MCP-1 TNF-Α 

分 类 号：R332[医药卫生—人体生理学]