瑶药“猛老虎”对免疫性肝纤维化大鼠的作用研究  

Study on Effect of Yao Nationality Herb Named Menglaohu on Immunity Hepatic Fibrosis in Rats

作　　者：钟鸣[1] 余胜民[1] 黄琳芸[1] 郭力城[1] 陈少锋[1] 杨东爱[1] 

机构地区：[1]广西民族医药研究所,广西南宁530001

出　　处：《实用预防医学》2010年第10期2032-2035,共4页Practical Preventive Medicine

基　　金：国家自然科学基金资助项目(30560185);广西"新世纪十百千人才工程"专项资金(224227)

摘　　要：目的观察猛老虎对大鼠免疫性肝纤维化的影响。方法采用猪血清腹腔注射建立大鼠免疫性肝纤维化模型,通过观察肝组织Ⅰ、Ⅲ型胶原、TGF-β1、Hyp、MMP-1和TIMP-1含量,血清PCⅢ、LN、HA、IFN-γ、TNF-α等指标的变化,以及各组大鼠肝组织的病理学改变,对猛老虎石油醚、乙酸乙酯、乙醇和水溶4个部位进行抗肝纤维化药效学试验筛选,以判断猛老虎具有抗肝纤维化活性作用的部位。结果猛老虎乙酸乙酯部位能减少大鼠血清PCⅢ、LN、HA和TNF-α含量,提高IFN-γ水平;减少大鼠肝组织Ⅰ、Ⅲ型胶原、TGF-β1相对表达和Hyp、TIMP-1含量,促进MMP-1生成,效果与秋水仙碱相当。结论猛老虎具有抗肝纤维化活性的有效部位主要存在于乙酸乙酯部位。Objective To study the effect of Menglaohu on immunity hepatic fibrosis in rats.Methods The model rats of immunity hepatic fibrosis were induced by intraperitoneal injection of porcine serum.Pharmacodynamic screening test was used to judge which part of Menglaohu（including the petroleum ether part,the ethyl acetate part,the ethylalcohol part and the water-dissolve part） had the effect of anti-hepatic fibrosis.The contents of col-Ⅰ,col-Ⅲ,TGF-β1,Hyp,MMP-1 and TIMP-1 in rats＇ liver tissues,the changes of PCⅢ,LN,HA,IFN-γ,and TNF-α in rats＇ blood sera,and the changes of histopathology in rats＇ livers were observed.Results The results showed that the ethyl acetate extract of Menglaohu could significantly decrease the contents of PCⅢ,LN,HA and TNF-α,increase the content of IFN-γ,down-regulate the expression of Ⅰ-and Ⅲ-type collagen and TGFβ1,the contents of Hyp and TIMP-1,and promote the level of MMP-1.Its effects were equivalent to those of the colchicine.Conclusions The effective part of anti-hepatic fibrosis of Menglaohu mainly exists in the ethyl acetate part.

关 键 词：猛老虎 大鼠 免疫性肝纤维化 药效学 

分 类 号：R332[医药卫生—人体生理学]