HCV人鼠嵌合体模型的制备初探  

Preliminary Investigation on Preparation of HCV Human-Mouse Chimeric Model

作　　者：周笛[1] 刘光泽[1] 范沛[1] 顾为望[1] 

机构地区：[1]南方医科大学实验动物中心暨比较医学研究所,广东广州510515

出　　处：《实用预防医学》2010年第10期2088-2090,共3页Practical Preventive Medicine

摘　　要：目的为制定人鼠嵌合HCV感染小鼠模型做初步研究。方法将人骨髓干细胞,经肝脏对KM小鼠胎鼠,每只移植1×106人骨髓干细胞。用放射免疫法对移植小鼠肝脏中的甲胎蛋白进行免疫组织化学检测,分析人肝细胞在小鼠体内生长情况。结果移植后五周内可检测到甲胎蛋白。结论将人骨髓干细胞移植小鼠肝脏内能够存活并分化成肝细胞。Objective To establish the human-mouse chimeric Fab against HCV.Methods The human bone marrow stem cells were transplanted into each mouse with the number of 1×10^6 via liver to fetal mouse in KM mouse.A radioimmunoassay（RIA） method was used to test the AFP in the transplanted mouse liver.The living condition of human liver cell in the mouse body was analyzed.Results Five weeks after transplantation,AFP was tested and within its normal value.Conclusions The human bone marrow stem cell can live and differentiate the liver cell after its transplantation into mouse liver.

关 键 词：HCV 人鼠嵌合 甲胎蛋白 

分 类 号：R332[医药卫生—人体生理学]