画眉草弯孢霉菌株QZ-2000与低剂量化学除草剂复配增效机制研究  

Synergy of the Mixture of Curvularia eragrostidis Strain QZ-2000 and Low-rate Herbicide to Digitaria sanguinalis Indicatedby Some Physiological Parameters

作　　者：王建书[1,2] 朱云枝[1] 赵敏[2] 强胜[1] 

机构地区：[1]南京农业大学杂草研究室,江苏南京210095 [2]河北工程大学农学院,河北邯郸056021

出　　处：《河南农业科学》2010年第10期65-67,70,共4页Journal of Henan Agricultural Sciences

基　　金：国家"863"计划项目(2001AA246012);国家自然科学基金(30170619);高校博士点基金(20050307002);江苏省自然科学基金(BK2001066)

摘　　要：画眉草弯孢霉菌株QZ-2000具有防除马唐等禾本科杂草的潜力,但是,对环境湿度具有依赖性,通过低剂量化学除草剂与其孢子复配,可以显著提高其活性和降低对环境的依赖性,但这种影响是由化学除草剂还是孢子起主导作用,仅从形态学方面往往难以判断。鉴此,通过对复配处理的马唐叶绿素、可溶性蛋白和丙二醛等生理指标的测定,明确这些生理指标的变化,进一步分析和判断复配增效作用机制。结果表明,各处理马唐叶绿素和可溶性蛋白含量呈下降趋势,丙二醛含量呈上升趋势。经孢子悬浮液与低剂量化学除草剂复配处理的马唐的叶绿素和可溶性蛋白含量下降幅度和丙二醛含量上升幅度最大,而低剂量化学除草剂处理的马唐生理指标变化幅度最小。表明画眉草弯孢霉菌株QZ-2000孢子与低剂量化学除草剂复配具有增效作用,且孢子起主导作用。Curvularia eragrostidis strain QZ-2000 has a potential for biological control of large crabgrass（Digitaria sanguinalis）.Its control efficacy,which is affected by environmental humidity conditions,can be improved and the dependence on the environment can be reduced through application of the mixture of Curvularia eragrostidis strain QZ-2000 conidia and low-rate herbicide.It is difficult to assess conidia or herbicide playing a main role in synergy only based on injured morphological symptoms of large crabgrass.The chlorophyll,soluble protein and MDA content in treated large crabgrass leaves were tested to enunciate the change of those physiological parameters and analyze the synergistic mechanism.The results showed chlorophyll and soluble protein content decreased and MDA content increased in all treatments.The mixture of conidia and low-rate herbicide demonstrated the most significant decrease of chlorophyll and soluble protein content while the most significant increase of MDA content.This indicated that there was synergy between conidia and low-rate herbicide,in which conidia played the predominant role.

关 键 词：画眉草弯孢霉菌株QZ-2000 叶绿素 可溶性蛋白 丙二醛 

分 类 号：S451[农业科学—植物保护] Q945.78[生物学—植物学]