榆耳多糖的分离纯化、结构鉴定及抗肿瘤活性研究  

Study on purification,structure identification and antitumor activity of polysaccharides from Gleoestereum incarnatum

作　　者：柳洪芳[1,2] 王新宇[1] 吕金超[1] 宋慧[1] 

机构地区：[1]吉林农业大学生命科学学院,吉林长春130118 [2]黑龙江省农垦科学院测试化验中心,黑龙江佳木斯154007

出　　处：《中国生化药物杂志》2010年第5期293-296,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家科技支撑计划(2007BAI38B06)

摘　　要：目的对榆耳粗多糖(GI)进行分离纯化,并对所得均一多糖进行结构鉴定及抗肿瘤活性的研究。方法 GI经Q-Sepharose F.F离子交换色谱,Sepharose CL-6B凝胶色谱分离得到均一多糖,采用凝胶色谱、薄层色谱、红外光谱、高碘酸氧化等方法测定相对分子质量(Mr)、单糖组成、糖苷键连接方式。采用MTT法对粗多糖和均一多糖的体外抗肿瘤活性进行测定。结果分离得到均一多糖GI1-3和GI1-4b,其Mr分别约为1.55×105和3.03×104。经薄层色谱法、红外光谱法、高碘酸氧化法确定GI1-3由半乳糖、葡萄糖、甘露糖、木糖组成,以β-(1,4)、β-(1,6)、β-(1,3)糖苷键连接。GI1-4b由葡萄糖、甘露糖和木糖组成,以β-(1,4)、β-(1,6)糖苷键连接。体外抗肿瘤实验表明,GI1-3的抑瘤活性较GI1-4b和GI高,GI的抑瘤活性在3种多糖组分中最低。结论 GI、GI1-3和GI1-4b对体外生长的肿瘤细胞具有不同的抑制作用,可能与其Mr及分子结构有关。Purpose Gleoestereum incarnatum crude polysaccharide was selected to be purified,to conduct structural identifiction and to carry on research on antitumor activity.Methods The homogeneous polysaccharides were obtained by the way of Q-Sepharose F.F and Sepharose CL-6B,and they were identificated by thin layer chromatography,infrared spectrometry and periodate oxidation method.The antitumor activity was determined by MTT methods.Results Molecular weights of GI1-3 and GI1-4b were about 1.55×105 Da and 3.03×104 Da.GI1-3 was made of galactose,glucose,mannose and xylose,linking to β-（1,4）,β-（1,6）,β-（1,3） glycosidic bond.GI1-4b was made of glucose,mannose and xylose linking to β-（1,4）,β-（1,6） glycosidic bond.The result of MTT showed that GI1-3 was the best component having antitumor activity,and GI was the worst one of three polysaccharides which we tested.Conclusion GI,GI1-3 and GI1-4b have different inhibiting effect on tumor cell in vitro,and this may be related to the difference of their molecular weight and molecular structure.

关 键 词：榆耳发酵浸膏 多糖 分离纯化 抗肿瘤 

分 类 号：R285[医药卫生—中药学] TQ464.1[医药卫生—中医学]