HPLC-ELSD法测定复方藏药制剂肝纤消颗粒中胆酸含量  

Determination of Cholic acid in Tibetan Medicine Preparation Ganxianxiao Granules by HPLC-ELSD

作　　者：徐梦丹[1] 鞠建明[1] 黄一平[1] 万元松[2] 

机构地区：[1]江苏省中医药研究院,南京210028 [2]南京工业大学,南京210009

出　　处：《中国实验方剂学杂志》2010年第13期39-40,共2页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省社会发展资助项目(BS2007089);拉萨市科技局重大科技项目(200414)

摘　　要：目的:测定复方藏药制剂肝纤消颗粒中胆酸的含量。方法:采用HPLC-ELSD法进行含量测定,色谱条件为色谱柱AlltimaC18(4.6 mm×250 mm,5μm);流动相乙腈-0.3%乙酸溶液(40∶60);流速1.0 mL.min-1;柱温35℃;ELSD漂移管温度100℃;载气流量2.6 L.min-1;进样量20μL,以外标两点对数方程计算样品的含量。结果:胆酸的线性范围为2.052～32.832μg,r=0.999 2,肝纤消颗粒样品中胆酸高、中、低浓度的加样回收率分别为97.1%,99.4%,97.4%,RSD分别为0.7%,0.4%,1.1%(n=3)。结论:该方法简便、快速、准确、重复性好,可用于控制肝纤消颗粒的质量。Objective： To establish a method for the determination of cholic acid in the compound preparation Ganxianxiao Granules.Method：HPLC-ELSD was used in the quantitative analysis with the condition as follows.Alltima C18（4.6 mm × 250 mm,5 μm） was used as column;mobile phase was acetonitrile-0.3% HAc（40∶ 60）;the temperature of column was at 35 ℃;flow rate was 1.0 mL·min^-1,the temperature of evaporator tube was set at 100 ℃;carrier gas flow was 2.6 L·min^-1 and the injection volume was 20 μL,calculating the content with logarithmic equation of external standard.Result：Cholic acid was linear in the range of 2.052～32.832 μg（r=0.999 2）.The average recovery of high,middle,low concentration in Ganxianxiao Granules was 97.1%,99.4%,97.4%,with RSD 0.7%,0.4%,1.1%（n = 3） respectively.Conclusion： This method was simple,reliable,accurate,and could be used to control the quality of the compound preparation Ganxianxiao Granules.

关 键 词：肝纤消颗粒 胆酸 高效液相色谱-漂移管阵列法 

分 类 号：R284.1[医药卫生—中药学]