HPLC测定独一味软胶囊中8-epideoxyloganic acid的含量  

Determination of 8-epideoxyloganic Acid in Duyiwei Soft Capsules by HPLC

作　　者：桑育黎[1] 郝延军[2] 陈沉[1] 徐嘉瞳[1] 

机构地区：[1]辽宁大学药学院,沈阳110036 [2]辽宁省食品药品检验所,沈阳110023

出　　处：《中国实验方剂学杂志》2010年第13期107-108,共2页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省教育厅资助项目(2009A313)

摘　　要：目的:建立独一味软胶囊中8-epideoxyloganic acid的含量测定方法。方法:采用高效液相色谱法。色谱柱:迪马C18(4.6 mm×250 mm,5μm),柱温40℃,流动相甲醇-0.025 mol·L-1磷酸水(50∶50),流速1.0 mL·min-1,检测波长238 nm。结果:8-epideoxyloganic acid进样量在1.979 2～9.896μg线性关系良好,回收率均在95.20%～100.00%,RSD均小于5%。结论:此方法准确可靠,重复性好,结果稳定,可作为独一味软胶囊及其他独一味制剂中环烯醚萜成分含量测定的方法。Objective： To set up a method for the determination of 8-epideoxyloganic acid in Duyiwei soft capsule.Method： The quantitative analysis was performed on Diamond C18（4.6 mm × 250 mm,59 μm） column and a mixture of methonal-0.025 mol·L-1 phosphate solution（50∶ 50） was used as the mobile phrase at a flow rate of 1.0 mL·min-1.The column temperature was at 40 ℃ and the detective wavelength was at 238 nm.Result： The linear range was 1.979 2～9.896 μg for 8-epideoxyloganic acid.The average recovery was 96.84% with RSD 2.07%.Conclusion： The method was accurate,reproducible,stable,and can be used as a quantitative standard for determination of iridoid glycosides in Duyiwei soft capsule.

关 键 词：独一味软胶囊 含量测定 8-epideoxyloganic ACID 

分 类 号：R284.1[医药卫生—中药学]