疏肝利胆法对染铅大鼠驱铅效应及拮抗海马损害的研究  

Shugan Lidan Therapy for Discharging Lead and Antagonizing Damage of Hippocampus in Lead-poisoned Rat

作　　者：张喜莲[1] 马融[1] 刘璇[1] 

机构地区：[1]天津中医药大学第一附属医院儿科,天津300193

出　　处：《中国实验方剂学杂志》2010年第13期122-126,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：天津市社会发展计划应用基础研究(05YFJMJC07900)

摘　　要：目的:探讨疏肝利胆法对染铅大鼠驱铅效应及拮抗海马损害的机制。方法:以醋酸铅灌胃制作铅中毒模型,实验大鼠随机分为疏肝利胆颗粒高、中、低剂量组,智杞颗粒组,依地酸钙钠组,模型对照组,空白对照组。采用等离子原子发射光谱法(ICP)测定各组大鼠治疗前后血中铅、钙、锌的浓度;电镜观察海马结构损伤;紫外分光光度法测定治后各组大鼠海马中一氧化氮(NO)和总抗氧化能力(TAOC)。结果:①疏肝利胆颗粒、智杞颗粒及依地酸钙钠3组治后血铅含量均明显降低,组间无显著差异;疏肝利胆颗粒中、高剂量对血钙的降低与智杞颗粒相当,明显低于依地酸钙钠,而对血锌的降低,显著低于智杞颗粒及依地酸钙钠。②染铅大鼠海马结构明显损伤,各治疗组中疏肝利胆组海马结构损伤最轻,其次是智杞颗粒、依地酸钙钠组。③染铅大鼠海马中NO及TAOC含量显著降低,疏肝利胆颗粒高剂量组海马中NO,TAOC含量明显增高,较其他治疗组有显著差异。结论:疏肝利胆法可降低血铅含量,避免必需微量元素钙、锌的流失;同时能拮抗铅所致海马损伤,提高NO及TAOC含量,改善学习记忆能力。Objective：To explore the effect of Shugan Lidan（SGLD） therapy for discharging lead and antagonizing the damage of hippocampus.Method：Lead poisoned model of rat was established by giving lead acetate ig for 5 weeks.All rats were randomly divided into seven groups：SGLD high-does group,SGLD middle-does group,SGLD low-does group,Zhiqi Granule group,EDTA-CaNa2 group,plumbum dyed model group and normal control group.We measured the concentrations of lead,calcium,zinc in blood by inductively coupled plasma atomic emission spectrometry（ICP）;observed morphological changes in hippocampus by electronic microscope;and measured the concentrations of NO,TAOC in the hippocampus cortex by UV spectrophotometry.Result： ① The treatments with SGLD,Zhiqi and EDTA-CaNa2 lowered the level of blood lead significantly,without significant differences among the three groups.Meanwhile the treatments caused the level of calcium and zinc in blood to drop,among the groups medium dose and high dose of SGLD dropped the level of calcium as much as Zhiqi,and the effect was lower than that of EDTA-CaNa2.However,blood zinc was observably dropped less by SGLD than that of Zhiqi and EDTA-CaNa2.② The hippocampus of lead-poisoned rat was injured obviously,but the damage in SGLD groups was the lightest.③ NO,TAOC in hippocampus of rat was explicitly lack in plumbum dyed model group.The high dose of SGLD could significantly increase the NO,TAOC in hippocampus.And It is better than those in the middle and low doses of SGLD,Zhiqi and EDTA-CaNa2.Conclusion：Shugan Lidan therapy can accelerate the elimination of lead,avoid loss of the endogenous essential elements calcium,zinc,meanwhile antagonize the damage of hippocampus,increase the level of NO,TAOC,and improve learning and memory ability.

关 键 词：疏肝利胆法 铅 必需微量元素 海马 

分 类 号：R285.5[医药卫生—中药学]